Title:
METHOD FOR DIFFERENTIATING PRIMORDIAL GERM CELLS INTO PRIMORDIAL FOLLICLES IN VITRO
Document Type and Number:
WIPO Patent Application WO/2019/244581
Kind Code:
A1
Abstract:
This method for differentiating primordial germ cells into primordial follicles in vitro involves culturing, under a pressurized condition or a low oxygen concentration condition, primordial germ cells and sustentacular cells adjoining the primordial germ cells.
Inventors:
NAGAMATSU GO (JP)
NISHIMURA YOHEI (JP)
SHIMAMOTO SO (JP)
HAYASHI KATSUHIKO (JP)
NISHIMURA YOHEI (JP)
SHIMAMOTO SO (JP)
HAYASHI KATSUHIKO (JP)
Application Number:
PCT/JP2019/021209
Publication Date:
December 26, 2019
Filing Date:
May 29, 2019
Export Citation:
Assignee:
UNIV KYUSHU NAT UNIV CORP (JP)
International Classes:
C12N5/075
Domestic Patent References:
| WO2017047799A1 | 2017-03-23 | |||
| WO2017047799A1 | 2017-03-23 |
Foreign References:
| JP2018117988A | 2018-08-02 |
Other References:
LOPEZ-IGLESIAS, P. ET AL.: "Hypoxia Induces Pluripotency in Primordial Germ Cells by HIF1 a Stabilization and Oct4 Deregulation", ANTIOXIDANTS & REDOX SIGNALING, vol. 22, no. 3, 20 January 2015 (2015-01-20), pages 205 - 223, XP055664444
MOROHAKU KANAKO, ET AL.: "Complete in vitro generation of fertile oocytes from mouse primordial germ cells", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 113, no. 32, 9 August 2016 (2016-08-09), pages 9021 - 9026, XP055664449
HIKABE, ORIE ET AL.: "Reconstitution in vitro of the entire cycle of the mouse female germ line", THE JOURNAL OF REPRODUCTION AND DEVELOPMENT SUPPLEMENT, 16 September 2016 (2016-09-16), pages 299 - 303, XP055664460, Retrieved from the Internet [retrieved on 20190807]
HIRAO, YUJI: "In vitro growth of oocytes", JOURNAL OF AZABU UNIVERSITY, vol. 29, 2017, pages 112, 113, XP009525415, ISSN: 1346-5880
MOROHAKU K. ET AL.: "Complete in vitro generation of fertile oocytes from mouse primordial germ cells", PROC NATL ACAD SCI, vol. 113, no. 32, 2016, pages 9021 - 9026, XP055664449, DOI: 10.1073/pnas.1603817113
OKITA K. ET AL.: "Generation of germline-competent induced pluripotent stem cells", NATURE, vol. 448, 2007, pages 313 - 317, XP055637413, DOI: 10.1038/nature05934
HAMANAKA S. ET AL.: "Generation of germline-competent rat induced pluripotent stem cells", PLOS ONE, vol. 6, 2011, pages e22008
HAYASHI K. ET AL.: "Reconstitution of the mouse germ cell specification pathway in culture by pluripotent stem cells", CELL, vol. 146, no. 4, 2011, pages 519 - 532, XP028383021, DOI: 10.1016/j.cell.2011.06.052
LI J ET AL.: "Activation of dormant ovarian follicles to generate mature eggs", PNAS., vol. 107, no. 22, 2010, pages 10280 - 10284
CAS , no. 663619-89-4
CAS, no. 371242-69-2
MOROHAKU KANAKO, ET AL.: "Complete in vitro generation of fertile oocytes from mouse primordial germ cells", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 113, no. 32, 9 August 2016 (2016-08-09), pages 9021 - 9026, XP055664449
HIKABE, ORIE ET AL.: "Reconstitution in vitro of the entire cycle of the mouse female germ line", THE JOURNAL OF REPRODUCTION AND DEVELOPMENT SUPPLEMENT, 16 September 2016 (2016-09-16), pages 299 - 303, XP055664460, Retrieved from the Internet
HIRAO, YUJI: "In vitro growth of oocytes", JOURNAL OF AZABU UNIVERSITY, vol. 29, 2017, pages 112, 113, XP009525415, ISSN: 1346-5880
MOROHAKU K. ET AL.: "Complete in vitro generation of fertile oocytes from mouse primordial germ cells", PROC NATL ACAD SCI, vol. 113, no. 32, 2016, pages 9021 - 9026, XP055664449, DOI: 10.1073/pnas.1603817113
OKITA K. ET AL.: "Generation of germline-competent induced pluripotent stem cells", NATURE, vol. 448, 2007, pages 313 - 317, XP055637413, DOI: 10.1038/nature05934
HAMANAKA S. ET AL.: "Generation of germline-competent rat induced pluripotent stem cells", PLOS ONE, vol. 6, 2011, pages e22008
HAYASHI K. ET AL.: "Reconstitution of the mouse germ cell specification pathway in culture by pluripotent stem cells", CELL, vol. 146, no. 4, 2011, pages 519 - 532, XP028383021, DOI: 10.1016/j.cell.2011.06.052
LI J ET AL.: "Activation of dormant ovarian follicles to generate mature eggs", PNAS., vol. 107, no. 22, 2010, pages 10280 - 10284
CAS , no. 663619-89-4
CAS, no. 371242-69-2
Attorney, Agent or Firm:
TANAI Sumio et al. (JP)
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