METHOD FOR ESTIMATING CELL COUNT, AND DEVICE FOR ESTIMATING CELL COUNT

Title:

METHOD FOR ESTIMATING CELL COUNT, AND DEVICE FOR ESTIMATING CELL COUNT

Document Type and Number:

WIPO Patent Application WO/2020/090225

Kind Code:

A1

Abstract:

[Problem] To provide a method and device for noninvasively estimating a cell count. [Solution] In the present invention, the concentration of a cell metabolite included in a culture medium for culturing certain cells is estimated using a simple method, e.g., using spectroscopy. The cell count of the certain cells can be estimated by fitting to relationship information obtained in advance indicating a relationship between the concentration of the cell metabolite and the cell count of the certain cells. In spectroscopy herein, because the actual concentration of a substance consumed by the cells can be estimated more accurately the higher the concentration of the cell metabolite is, the concentration of a substance consumed by the cells, which decreases over time over the course of culturing of the cells, is estimated in the initial-to-intermediate stage of cell culturing, and the concentration of a cell product which increases over time over the course of culturing of the cells is estimated in the intermediate-to-late stage of cell culturing. The cell count can thereby be estimated through the entire range from the initial stage to the ending of cell culturing.

Inventors:

SAKURAI TSUBASA
TAIRA KEISUKE

Application Number:

PCT/JP2019/034982

Publication Date:

May 07, 2020

Filing Date:

September 05, 2019

Export Citation:

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Assignee:

PHC HOLDINGS CORP (JP)

International Classes:

C12Q1/06; C12M1/00

Foreign References:

JP2010081809A	2010-04-15
JP2012520078A	2012-09-06
JP2014045663A	2014-03-17

Other References: