Title:
METHOD FOR EVALUATING CYP3A ENZYMATIC ACTIVITY IN HUMAN BODY (PHENOTYPING TEST METHOD)
Document Type and Number:
WIPO Patent Application WO/2016/199702
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a novel method for evaluating a CYP3A enzymatic activity in a human body (a phenotyping test method). The present invention relates to a method for evaluating a CYP3A enzymatic activity, in which the ratio of the concentration of a substrate involved in a CYP3A enzymatic reaction to the concentration of a product of the CYP3A enzymatic reaction in blood collected from a subject is determined as a measure for the CYP3A enzymatic activity.
Inventors:
SHIBASAKI HIROMI (JP)
FURUTA TAKASHI (JP)
YOKOKAWA AKITOMO (JP)
HIRANO RYOHEI (JP)
FURUTA TAKASHI (JP)
YOKOKAWA AKITOMO (JP)
HIRANO RYOHEI (JP)
Application Number:
PCT/JP2016/066683
Publication Date:
December 15, 2016
Filing Date:
June 03, 2016
Export Citation:
Assignee:
TOKYO UNIV OF PHARMACY & LIFE SCIENCES (JP)
International Classes:
C12Q1/26; G01N33/50
Foreign References:
| JP2007132741A | 2007-05-31 | |||
| JP2008275425A | 2008-11-13 | |||
| US20040022728A1 | 2004-02-05 |
Other References:
DAMKIER, PER ET AL.: "Quinidine as a probe for CYP3A4 activity: intrasubject variability and lack of correlation with probe-based assays for CYP1A2, CYP2C9, CYP2C19, and CYP2D6", CLIN. PHARMACOL. THER., vol. 68, no. 2, 2000, pages 199 - 209, XP055334617
BJORKHEM-BERGMAN, LINDA ET AL.: "Quinine compared to 4p-hydroxycholesterol and midazolam as markers for CYP3A induction by rifampicin, Drug Metab.", PHARMACOKINET, vol. 29, 2014, pages 352 - 355
HIROMI SHIBASAKI: "Taylor Made Yakubutsu Chiryo o Mezashite: CYP3A Kassei Hyoka kara no Approach", JAPANESE JOURNAL OF THERAPEUTIC DRUG MONITORING, vol. 30, 2013, pages 14 - 17
YAMASHITA, KOUWA ET AL.: "Simultaneous determination of tetrahydrocortisol, allotetrahydrocortisol and tetrahydrocortisone in human urine by liquid chromatography- electrospray ionization tandem mass spectrometry", STEROIDS, vol. 73, no. 7, 2008, pages 727 - 737, XP022673873
NAKAGAWA, YUMIKO ET AL.: "Polar Derivatization of 5a-Dihydrotestosterone and Sensitive Analysis by Semimicro-LC/ESI-MS", J. MASS SPECTROM. SOC. JPN., vol. 50, no. 6, 2002, pages 330 - 336, XP055334625
HIGASHI, TATSUYA ET AL.: "2-hydrazino-1- methylpyridine: a highly sensitive derivatization reagent for oxosteroids in liquid chromatography-electrospray ionization- mass spectrometry", J. CHROMATOGR. B, vol. 825, no. 2, 2005, pages 214 - 222, XP027627036
HIGASHI, TATSUYA ET AL.: "Procedure for increasing the detection responses of estrogens in LC-MS based on introduction of a nitrobenzene moiety followed by electron capture atmospheric pressure chemical ionization", ANAL. BIOANAL. CHEM., vol. 386, no. 3, 2006, pages 658 - 665, XP019441068
SINGH, GURKEERAT ET AL.: "Liquid Chromatography/ Electron Capture Atmospheric Pressure Chemical Ionization/Mass Spectrometry: Analysis of Pentafluorobenzyl Derivatives of Biomolecules and Drugs in the Attomole Range", ANAL. CHEM., vol. 72, no. 14, 2000, pages 3007 - 3013, XP002998461
NELSON, ROBERT E. ET AL.: "Liquid chromatography-tandem mass spectrometry assay for simultaneous measurement of estradiol and estrone in human plasma", CLIN. CHEM., vol. 50, no. 2, 2004, pages 373 - 384, XP002622663
SHIN, K.-H. ET AL.: "Evaluation of endogenous metabolic markers of hepatic CYP3A activity using metabolic profiling and midazolam clearance", CLIN. PHARMACOL. THER., vol. 94, no. 5, 2013, pages 601 - 609, XP055334656
FURUTA, TAKASHI ET AL.: "Evidence for the validity of cortisol 6 beta-hydroxylation clearance as a new index for in vivo cytochrome P450 3A phenotyping in humans", DRUG. METAB. DISPOS., vol. 31, no. 11, 2003, pages 1283 - 1287, XP055334662
RYOHEI HIRANO: "Kecchu 6P-Hydroxycortisol/ Kecchu Cortisol Nodohi ni Motozuku Shinki Hito in vivo CYP3A Kassei Hyokaho", JAPANESE JOURNAL OF THERAPEUTIC DRUG MONITORING, vol. 33, no. 2, 10 May 2016 (2016-05-10)
BJORKHEM-BERGMAN, LINDA ET AL.: "Quinine compared to 4p-hydroxycholesterol and midazolam as markers for CYP3A induction by rifampicin, Drug Metab.", PHARMACOKINET, vol. 29, 2014, pages 352 - 355
HIROMI SHIBASAKI: "Taylor Made Yakubutsu Chiryo o Mezashite: CYP3A Kassei Hyoka kara no Approach", JAPANESE JOURNAL OF THERAPEUTIC DRUG MONITORING, vol. 30, 2013, pages 14 - 17
YAMASHITA, KOUWA ET AL.: "Simultaneous determination of tetrahydrocortisol, allotetrahydrocortisol and tetrahydrocortisone in human urine by liquid chromatography- electrospray ionization tandem mass spectrometry", STEROIDS, vol. 73, no. 7, 2008, pages 727 - 737, XP022673873
NAKAGAWA, YUMIKO ET AL.: "Polar Derivatization of 5a-Dihydrotestosterone and Sensitive Analysis by Semimicro-LC/ESI-MS", J. MASS SPECTROM. SOC. JPN., vol. 50, no. 6, 2002, pages 330 - 336, XP055334625
HIGASHI, TATSUYA ET AL.: "2-hydrazino-1- methylpyridine: a highly sensitive derivatization reagent for oxosteroids in liquid chromatography-electrospray ionization- mass spectrometry", J. CHROMATOGR. B, vol. 825, no. 2, 2005, pages 214 - 222, XP027627036
HIGASHI, TATSUYA ET AL.: "Procedure for increasing the detection responses of estrogens in LC-MS based on introduction of a nitrobenzene moiety followed by electron capture atmospheric pressure chemical ionization", ANAL. BIOANAL. CHEM., vol. 386, no. 3, 2006, pages 658 - 665, XP019441068
SINGH, GURKEERAT ET AL.: "Liquid Chromatography/ Electron Capture Atmospheric Pressure Chemical Ionization/Mass Spectrometry: Analysis of Pentafluorobenzyl Derivatives of Biomolecules and Drugs in the Attomole Range", ANAL. CHEM., vol. 72, no. 14, 2000, pages 3007 - 3013, XP002998461
NELSON, ROBERT E. ET AL.: "Liquid chromatography-tandem mass spectrometry assay for simultaneous measurement of estradiol and estrone in human plasma", CLIN. CHEM., vol. 50, no. 2, 2004, pages 373 - 384, XP002622663
SHIN, K.-H. ET AL.: "Evaluation of endogenous metabolic markers of hepatic CYP3A activity using metabolic profiling and midazolam clearance", CLIN. PHARMACOL. THER., vol. 94, no. 5, 2013, pages 601 - 609, XP055334656
FURUTA, TAKASHI ET AL.: "Evidence for the validity of cortisol 6 beta-hydroxylation clearance as a new index for in vivo cytochrome P450 3A phenotyping in humans", DRUG. METAB. DISPOS., vol. 31, no. 11, 2003, pages 1283 - 1287, XP055334662
RYOHEI HIRANO: "Kecchu 6P-Hydroxycortisol/ Kecchu Cortisol Nodohi ni Motozuku Shinki Hito in vivo CYP3A Kassei Hyokaho", JAPANESE JOURNAL OF THERAPEUTIC DRUG MONITORING, vol. 33, no. 2, 10 May 2016 (2016-05-10)
Attorney, Agent or Firm:
ONO Makoto et al. (JP)
Makoto Ono (JP)
Makoto Ono (JP)
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