Title:
METHOD FOR IDENTIFYING THE AGONISTIC ACTIVITY OF A TARGET COMPOUND ON A POTASSIUM CHANNEL
Document Type and Number:
WIPO Patent Application WO2008110247
Kind Code:
A8
Abstract:
The invention relates to a method for identifying the agonistic activity of a target compound on a potassium channel, characterized in that: a) a population of cells is prepared, which express a potassium channel and optionally a protein-based fluorescent-optical strain sensor, b) optionally the cells are incubated according to a) with a strain-sensitive fluorescent dye, c) the target compound is added to the reaction mixture of a) or b), d) a value F1 is determined for the fluorescence intensity of the cells, d) potassium ions are added in a physiologically tolerable concentration, f) a value F2 is determined for the fluorescence intensity of the cells, and g) the fluorescence intensity F2 is compared with the fluorescence intensity F1, and the agonistic activity of the target compound on the potassium channel is determined therefrom. The invention also relates to a purified potassium channel agonist isolated with this method, medications containing said agonist, and the use thereof for treatment of a disease in which potassium channels are involved.
Inventors:
STRUENKER TIMO (DE)
CAVALAR MARKUS (DE)
BAHRENBERG GREGOR (DE)
CAVALAR MARKUS (DE)
BAHRENBERG GREGOR (DE)
Application Number:
PCT/EP2008/001161
Publication Date:
November 13, 2008
Filing Date:
February 15, 2008
Export Citation:
Assignee:
GRUENENTHAL GMBH (DE)
STRUENKER TIMO (DE)
CAVALAR MARKUS (DE)
BAHRENBERG GREGOR (DE)
STRUENKER TIMO (DE)
CAVALAR MARKUS (DE)
BAHRENBERG GREGOR (DE)
International Classes:
G01N33/58; G01N33/68
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