Title:
METHOD FOR IDENTIFYING CELLS CAUSING PERIODONTITIS, METHOD FOR SCREENING PERIODONTITIS THERAPEUTICS, METHOD FOR TESTING PERIODONTITIS, AND TEST KIT
Document Type and Number:
WIPO Patent Application WO/2016/152554
Kind Code:
A1
Abstract:
Established are an analysis of markers of aggressive fibroblasts which are cells that cause periodontitis, screening of novel periodontitis therapeutics, a test method, and a test kit. As a result of analyzing aggressive fibroblasts, it has become apparent that expression from the P1 promoter of RUNX2 and expression of DLX5 are decreased to a greater extent than in normal gingival fibroblasts. An increase in expression of FLT1 was also seen. Using these expressions characteristic of aggressive fibroblasts as markers makes possible the identification of aggressive fibroblasts, a method for screening therapeutics, and a test method.
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Inventors:
OHSHIMA MITSUHIRO (JP)
YAMAGUCHI YOKO (JP)
HORIE MASAFUMI (JP)
SAITO AKIRA (JP)
NAGASE TAKAHIDE (JP)
IWAMOTO HISAMI (JP)
YAMAGUCHI YOKO (JP)
HORIE MASAFUMI (JP)
SAITO AKIRA (JP)
NAGASE TAKAHIDE (JP)
IWAMOTO HISAMI (JP)
Application Number:
PCT/JP2016/057552
Publication Date:
September 29, 2016
Filing Date:
March 10, 2016
Export Citation:
Assignee:
I C ELECTRONICS CO LTD (JP)
UNIV NIHON (JP)
UNIV TOKYO (JP)
UNIV NIHON (JP)
UNIV TOKYO (JP)
International Classes:
C12Q1/68; G01N33/53; C12N15/09
Foreign References:
| JP2013216589A | 2013-10-24 |
Other References:
MITSUHIRO OSHIMA ET AL.: "Paradigm shift in pharmacological treatment of periodontitis", FOLIA PHARMACOLOGICA JAPONICA, vol. 141, 2013, pages 314 - 320
MITSUHIRO OSHIMA ET AL.: "Atarashii Shishuen nc Mikata to Tainshisei Shikkan Hassho no BINGO Model Kasetsu", RESEARCH ON DRUG INTERACTIONS, vol. 38, 2014, pages 82 - 88
DATABASE GenBank 6 December 2004 (2004-12-06), "DEFINITION: Homo sapiens vascular endothelial growth factor receptor (FLT1) mRNA, complete cds", XP055316273, Database accession no. AF063657
OHSHIMA, MITSUHIRO ET AL.: "Fibroblast VEGF- receptor 1 expression as molecular target in periodontitis", J. CLIN. PERIODONTOL., vol. 43, 23 February 2016 (2016-02-23), pages 128 - 137, XP055316265
ZHENG, WEI ET AL.: "Periodontitis promotes the proliferation and suppresses the differentiation potential of human periodontal ligament stem cells", INT. J. MOL. MED., vol. 36, 2015, pages 915 - 922, XP055316270, [retrieved on 20150817]
KOMORI, TOSHIHISA ET AL.: "Cbfa1 in bone development", CURR. OPIN. GENET. DEV., vol. 8, 1998, pages 494 - 499, XP004641200
MITSUHIRO OSHIMA ET AL.: "Atarashii Shishuen nc Mikata to Tainshisei Shikkan Hassho no BINGO Model Kasetsu", RESEARCH ON DRUG INTERACTIONS, vol. 38, 2014, pages 82 - 88
DATABASE GenBank 6 December 2004 (2004-12-06), "DEFINITION: Homo sapiens vascular endothelial growth factor receptor (FLT1) mRNA, complete cds", XP055316273, Database accession no. AF063657
OHSHIMA, MITSUHIRO ET AL.: "Fibroblast VEGF- receptor 1 expression as molecular target in periodontitis", J. CLIN. PERIODONTOL., vol. 43, 23 February 2016 (2016-02-23), pages 128 - 137, XP055316265
ZHENG, WEI ET AL.: "Periodontitis promotes the proliferation and suppresses the differentiation potential of human periodontal ligament stem cells", INT. J. MOL. MED., vol. 36, 2015, pages 915 - 922, XP055316270, [retrieved on 20150817]
KOMORI, TOSHIHISA ET AL.: "Cbfa1 in bone development", CURR. OPIN. GENET. DEV., vol. 8, 1998, pages 494 - 499, XP004641200
Attorney, Agent or Firm:
SHIRAKATA YUMIKO (JP)
White form Yumiko (JP)
White form Yumiko (JP)
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