Title:
METHOD FOR IDENTIFYING PROTEIN THAT IS UBIQUITINATED VIA TARGETED PROTEIN DEGRADATION INDUCER
Document Type and Number:
WIPO Patent Application WO/2023/068278
Kind Code:
A1
Abstract:
Provided is a method for identifying a protein that is ubiquitinated via a targeted protein degradation inducer, the method comprising: (1) a step for causing a probe that includes an E3 ligase part and a ubiquitin-binding part to act on a cell or an extract thereof in the presence of a targeted protein degradation inducer; and (2) a step for identifying a ubiquitinated protein that has bonded to the probe.
Inventors:
HATAKEYAMA SHIGETSUGU (JP)
WATANABE MASASHI (JP)
WATANABE MASASHI (JP)
Application Number:
PCT/JP2022/038798
Publication Date:
April 27, 2023
Filing Date:
October 18, 2022
Export Citation:
Assignee:
UNIV HOKKAIDO NAT UNIV CORP (JP)
International Classes:
C12Q1/02; C07K14/47; C12N15/12; C12Q1/00; C12Q1/68; G01N33/53
Domestic Patent References:
| WO2015072507A1 | 2015-05-21 | |||
| WO2013012038A1 | 2013-01-24 |
Other References:
WATANABE MASASHI, SAEKI YASUSHI, TAKAHASHI HIDEHISA, OHTAKE FUMIAKI, YOSHIDA YUKIKO, KASUGA YUSUKE, KONDO TAKESHI, YAGUCHI HIROAKI: "A substrate-trapping strategy to find E3 ubiquitin ligase substrates identifies Parkin and TRIM28 targets", COMMUNICATIONS BIOLOGY, vol. 3, no. 1, XP093057895, DOI: 10.1038/s42003-020-01328-y
WATANABE, MASASHI: "Research for Development of Novel Method of Comprehensive and Quantitative In-Cell Measurement of Ubiquitin ligase Substrates", ANNUAL REPORT, NAKATANI FOUNDATION FOR ADVANCEMENT OF MEASURING TECHNOLOGIES IN BIOMEDICAL ENGINEERING, no. 31, 20 March 2018 (2018-03-20), pages 204 - 210, XP009546711
WATANABE, MASASHI, HATAKEYAMA, SHIGETSUGU: "TRIM family proteins and targeted protein degradation", THE CELL, vol. 53, no. 3, 20 March 2021 (2021-03-20), pages 160 - 163, XP009545406
NAITO MIKIHIKO, NOBUMICHI OHOKA, NORIHITO SHIBATA, TAKAYUKI HATTORI: "Chemical protein knockdown: Development of SNIPER compounds that induce degradation of target proteins", MEDCHEM NEWS, vol. 28, no. 1, 1 January 2018 (2018-01-01), pages 29 - 35, XP093057918
XIUYUN SUN, GAO HONGYING, YANG YIQING, HE MING, WU YUE, SONG YUGANG, TONG YAN, RAO YU: "PROTACs: great opportunities for academia and industry", SIGNAL TRANSDUCTION AND TARGETED THERAPY, vol. 4, no. 1, 1 December 2019 (2019-12-01), pages 1 - 33, XP055767841, DOI: 10.1038/s41392-019-0101-6
YOSHIDA YUKIKO, SAEKI YASUSHI, MURAKAMI ARISA, KAWAWAKI JUNKO, TSUCHIYA HIKARU, YOSHIHARA HIDEHITO, SHINDO MAYUMI, TANAKA KEIJI: "A comprehensive method for detecting ubiquitinated substrates using TR-TUBE", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 112, no. 15, 14 April 2015 (2015-04-14), pages 4630 - 4635, XP093057924, ISSN: 0027-8424, DOI: 10.1073/pnas.1422313112
WATANABE, MASASHI: "Research for Development of Novel Method of Comprehensive and Quantitative In-Cell Measurement of Ubiquitin ligase Substrates", ANNUAL REPORT, NAKATANI FOUNDATION FOR ADVANCEMENT OF MEASURING TECHNOLOGIES IN BIOMEDICAL ENGINEERING, no. 31, 20 March 2018 (2018-03-20), pages 204 - 210, XP009546711
WATANABE, MASASHI, HATAKEYAMA, SHIGETSUGU: "TRIM family proteins and targeted protein degradation", THE CELL, vol. 53, no. 3, 20 March 2021 (2021-03-20), pages 160 - 163, XP009545406
NAITO MIKIHIKO, NOBUMICHI OHOKA, NORIHITO SHIBATA, TAKAYUKI HATTORI: "Chemical protein knockdown: Development of SNIPER compounds that induce degradation of target proteins", MEDCHEM NEWS, vol. 28, no. 1, 1 January 2018 (2018-01-01), pages 29 - 35, XP093057918
XIUYUN SUN, GAO HONGYING, YANG YIQING, HE MING, WU YUE, SONG YUGANG, TONG YAN, RAO YU: "PROTACs: great opportunities for academia and industry", SIGNAL TRANSDUCTION AND TARGETED THERAPY, vol. 4, no. 1, 1 December 2019 (2019-12-01), pages 1 - 33, XP055767841, DOI: 10.1038/s41392-019-0101-6
YOSHIDA YUKIKO, SAEKI YASUSHI, MURAKAMI ARISA, KAWAWAKI JUNKO, TSUCHIYA HIKARU, YOSHIHARA HIDEHITO, SHINDO MAYUMI, TANAKA KEIJI: "A comprehensive method for detecting ubiquitinated substrates using TR-TUBE", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 112, no. 15, 14 April 2015 (2015-04-14), pages 4630 - 4635, XP093057924, ISSN: 0027-8424, DOI: 10.1073/pnas.1422313112
Attorney, Agent or Firm:
KOBAYASHI Hiroshi et al. (JP)
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