Title:
METHOD FOR IMPROVING EXPRESSION LEVEL OF RECOMBINANT HUMAN ALBUMIN, AND CELL AND PROTEIN
Document Type and Number:
WIPO Patent Application WO/2024/045153
Kind Code:
A1
Abstract:
Provided is a method for improving the expression level of a recombinant human albumin, which comprises expressing the recombinant human albumin, and weakening, destroying or removing the function of at least one endogenous gene encoding a flocculating protein, and/or editing an endogenous gene or heterologous gene to overexpress superoxide dismutase; and/or editing an endogenous gene or heterologous gene to overexpress E3 ubiquitin ligase. Further provided are a corresponding cell and albumin.
Inventors:
XIANG WEI (CN)
HAN XU (CN)
HAN XU (CN)
Application Number:
PCT/CN2022/116713
Publication Date:
March 07, 2024
Filing Date:
September 02, 2022
Export Citation:
Assignee:
TONGHUA ANRATE BIOPHARMACEUTICAL CO LTD (CN)
International Classes:
C07K14/765; C12N1/19; C12N15/14; C12N15/53; C12N15/81; C12R1/72; C12R1/78; C12R1/84
Foreign References:
| CN111363688A | 2020-07-03 | |||
| CN103194481A | 2013-07-10 | |||
| CN103468595A | 2013-12-25 | |||
| CN103773793A | 2014-05-07 | |||
| CN103906833A | 2014-07-02 | |||
| CN1150457A | 1997-05-21 | |||
| CN107778365A | 2018-03-09 | |||
| CN1239103A | 1999-12-22 |
Attorney, Agent or Firm:
SHANGHAI ZHISHENG INTELLECTUAL PROPERTY OFFICE (CN)
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