Title:
METHOD AND KIT FOR DETECTING HUMAN α-DEFENSIN HD5, AND ANTIBODIES USED IN SAID METHOD AND KIT
Document Type and Number:
WIPO Patent Application WO/2021/149798
Kind Code:
A1
Abstract:
[Problem] The purpose of the present invention is to provide a method for detecting, in a selective and simple manner, an oxidized form of HD5 which is human α-defensin, that is, HD5 having intramolecular disulfide bonds that function beneficially in the human body. [Solution] The present invention relates to: two kinds of novel monoclonal antibodies that contain amino acid sequences of SEQ ID NO: 1-6 or 7-12 as a CDR and that specifically bind to human α-defensin HD5 having intramolecular disulfide bonds; an immunological detection method using the antibodies; and a kit containing the antibodies.
Inventors:
AYABE TOKIYOSHI (JP)
NAKAMURA KIMINORI (JP)
NAKAMURA KIMINORI (JP)
Application Number:
PCT/JP2021/002250
Publication Date:
July 29, 2021
Filing Date:
January 22, 2021
Export Citation:
Assignee:
UNIV HOKKAIDO NAT UNIV CORP (JP)
International Classes:
C07K16/18; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/13; C12N15/63; G01N33/53; G01N33/531; G01N33/543
Other References:
PORTER, E. M. ET AL.: "Localization of Human Intestinal Defensin 5 in Paneth Cell Granules", INFECTION AND IMMUNITY, vol. 65, no. 6, 1997, pages 2389 - 2395, XP055843420
SPENCER, J. D. ET AL.: "Human Alpha Defensin 5 Expression in the Human Kidney and Urinary Tract", PLOS ONE, vol. 7, no. 2, 16 February 2012 (2012-02-16), pages e31712, XP055843428
NAKAMURA, KIMINORI ET AL.: "A monoclonal antibody- based sandwich enzyme-linked immunosorbentassay for detection of secreted a-defensin", ANALYTICAL BIOCHEMISTRY, vol. 443, 2013, pages 124 - 131, XP055843428
DING, BENJAMIN TK ET AL.: "Accuracy of the a- defensin lateral flow assay for diagnosing periprosthetic joint infection in Asians", JOURNAL OF ORTHOPAEDIC SURGERY, vol. 27, no. 1, 2019, pages 1 - 9, XP055843430
WOMMACK, A. J. ET AL.: "NMR solution structure and condition-dependent oligomerization of the antimicrobial peptide human defensin 5", BIOCHEMISTRY, vol. 51, no. 48, 19 November 2012 (2012-11-19), pages 9624 - 9637, XP055843431
NAKAMURA, KIMINORI ET AL.: "Regulation of the Intestinal Microbiota by Antimicrobial Peptide a-Defensin", JOURNAL OF INTESTINAL MICROBIOLOGY, vol. 33, no. 3, 2019, pages 129 - 135, XP055843433
K. MASUDA ET AL., J. INNATE IMMUN., vol. 3, 2011, pages 315 - 326
K. NAKAMURA ET AL., ANALYTICAL BIOCHEMISTRY., vol. 443, no. 2, 2013, pages 124 - 131
MEYAARD ET AL., IMMUNITY, vol. 7, 1997, pages 283 - 290
WRIGHT ET AL., IMMUNITY, vol. 13, 2000, pages 233 - 242
KAITHAMANA ET AL., J.IMMUNOL., vol. 163, 1999, pages 5157 - 5164
K. NAKAMURA ET AL., ANALYTICAL BIOCHEMISTRY, vol. 443, no. 2, 2013, pages 124 - 131
See also references of EP 4011912A4
SPENCER, J. D. ET AL.: "Human Alpha Defensin 5 Expression in the Human Kidney and Urinary Tract", PLOS ONE, vol. 7, no. 2, 16 February 2012 (2012-02-16), pages e31712, XP055843428
NAKAMURA, KIMINORI ET AL.: "A monoclonal antibody- based sandwich enzyme-linked immunosorbentassay for detection of secreted a-defensin", ANALYTICAL BIOCHEMISTRY, vol. 443, 2013, pages 124 - 131, XP055843428
DING, BENJAMIN TK ET AL.: "Accuracy of the a- defensin lateral flow assay for diagnosing periprosthetic joint infection in Asians", JOURNAL OF ORTHOPAEDIC SURGERY, vol. 27, no. 1, 2019, pages 1 - 9, XP055843430
WOMMACK, A. J. ET AL.: "NMR solution structure and condition-dependent oligomerization of the antimicrobial peptide human defensin 5", BIOCHEMISTRY, vol. 51, no. 48, 19 November 2012 (2012-11-19), pages 9624 - 9637, XP055843431
NAKAMURA, KIMINORI ET AL.: "Regulation of the Intestinal Microbiota by Antimicrobial Peptide a-Defensin", JOURNAL OF INTESTINAL MICROBIOLOGY, vol. 33, no. 3, 2019, pages 129 - 135, XP055843433
K. MASUDA ET AL., J. INNATE IMMUN., vol. 3, 2011, pages 315 - 326
K. NAKAMURA ET AL., ANALYTICAL BIOCHEMISTRY., vol. 443, no. 2, 2013, pages 124 - 131
MEYAARD ET AL., IMMUNITY, vol. 7, 1997, pages 283 - 290
WRIGHT ET AL., IMMUNITY, vol. 13, 2000, pages 233 - 242
KAITHAMANA ET AL., J.IMMUNOL., vol. 163, 1999, pages 5157 - 5164
K. NAKAMURA ET AL., ANALYTICAL BIOCHEMISTRY, vol. 443, no. 2, 2013, pages 124 - 131
See also references of EP 4011912A4
Attorney, Agent or Firm:
IP-ASSIST PATENT OFFICE (JP)
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