SERUM-FREE MEDIUM AND CULTURING METHOD SUITED FOR CULTURING BLOOD CELLS SUCH AS HUMAN HEMATOPOIETIC STEM CELLS

WIPO Patent Application WO/2021/149799

A1

The present invention discloses a composition of a serum-free medium and a culturing method suited for culturing human cells. The present invention provides a method for culturing human cells. The method comprises bringing human cells into contact with a polyalkylene glycol modified by a copolymer of a polyvinyl caprolactam block and a polyvinyl acetate block.

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YAMAZAKI SATOSHI (JP)
WATANABE MOTOO (JP)

PCT/JP2021/002252

July 29, 2021

January 22, 2021

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UNIV TOKYO (JP)

C12N5/0789

WO2013059357A1

2013-04-25

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WILKINSON, A. C. ET AL.: "Long-term ex vivo haematopoietic-stem- cell expansion allows nonconditioned transplantation", NATURE, vol. 571, 2019, pages 117 - 121, XP036970458, DOI: 10.1038/s41586-019-1244-x
WILKINSON, A. ET AL.: "Long-term ex vivo expansion of functional hematopoietic stem cells", EXPERIMENTAL HEMATOLOGY, vol. 76, 2019, pages S42, XP085790831, DOI: 10.1016/j.exphem.2019.06.276
WILKINSON ET AL., NATURE, vol. 571, 2019, pages 117 - 121
"GenBank", Database accession no. AAA85450.1

OHNO Seiji et al. (JP)

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