Title:
METHOD FOR MANUFACTURING CELL SUSPENSION AND METHOD FOR MANUFACTURING ADHERENT CELL
Document Type and Number:
WIPO Patent Application WO/2021/181819
Kind Code:
A1
Abstract:
One embodiment of this invention relates to a method for manufacturing a cell suspension including (A), (B) and (C). (A) culturing adherent cells in a cell suspension containing the adherent cells, a microcarrier, and a medium and having a volume of 0.3 L or more; (B) culturing the adherent cells in a cell suspension containing the adherent cells, the microcarrier, and the medium obtained by (A) above and having a volume of 5 L or more; and (C) culturing the adherent cells in a cell suspension containing the adherent cells, the microcarrier, and the medium obtained by (B) above and having a volume of 10 L or more.
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Inventors:
CHEN SHANGWU (JP)
NAKAGAWA FUMIKO (JP)
TADA YASUHIKO (JP)
OKANOJO MASAHIRO (JP)
SATO YUSHI (JP)
SUZUKI YUMA (JP)
NAKASHIMA KATSUHIKO (JP)
TAKAHASHI RYOSUKE (JP)
NAKAGAWA FUMIKO (JP)
TADA YASUHIKO (JP)
OKANOJO MASAHIRO (JP)
SATO YUSHI (JP)
SUZUKI YUMA (JP)
NAKASHIMA KATSUHIKO (JP)
TAKAHASHI RYOSUKE (JP)
Application Number:
PCT/JP2020/047981
Publication Date:
September 16, 2021
Filing Date:
December 22, 2020
Export Citation:
Assignee:
SHOWA DENKO MATERIALS CO LTD (JP)
International Classes:
C12N5/071; C12N5/0775
Domestic Patent References:
| WO2009105044A1 | 2009-08-27 |
Foreign References:
| JP2008515412A | 2008-05-15 | |||
| JP2020043974A | 2020-03-26 |
Other References:
CHIN, S. B.: "P02-113: 50L large scale culture of mesenchymal stem cells for regenerative medicine", REGENERATIVE MEDICINE, vol. 19, 12 March 2020 (2020-03-12) - 14 March 2020 (2020-03-14), JP, pages 795, XP009531401, ISSN: 1347-7919
ADLERZ K., TREMPEL M., WANG D., KIRIAN R.D., ROWLEY J.A., AHSAN T.: "Comparison of msc-evs manufatured in 2D versus scalable 3D bioreactor systems", CYTOTHERAPY, ISIS MEDICAL MEDIA, OXFORD,, GB, vol. 21, no. 5, 1 May 2019 (2019-05-01), GB , pages S58, XP055859057, ISSN: 1465-3249, DOI: 10.1016/j.jcyt.2019.03.434
DE SOURE, A. M. ET AL.: "Scalable microcarrier- based manufacturing of mesenchymal stem/stromal cells", JOURNAL OF BIOTECHNOLOGY, vol. 236, 2016, pages 88 - 109, XP029729470, DOI: 10.1016/j.jbiotec.2016.08.007
CHEN, A. K. L. ET AL.: "Application of human mesenchymal and pluripotent stem cell microcarrier cultures in cellular therapy: Achievements and future direction", BIOTECHNOLOGY ADVANCES, vol. 31, 2013, pages 1032 - 1046, XP055113967, DOI: 10.1016/j.biotechadv.2013.03.006
LAWSON, T. ET AL.: "Process development for expansion of human mesenchymal stromal cells in a 50 L single-use stirred tank bioreactor", BIOCHEMICAL ENGINEERING JOURNAL, vol. 120, 2017, pages 49 - 62, XP029932798, DOI: 10.1016/j.bej.2016.11.020
RAFIQ, Q. A. ET AL.: "Qualitative and quantitative demonstration of bead-to-bead transfer with bone marrow-derived human mesenchymal stem cells on microcarriers: Utilising the phenomenon to improve culture performance", BIOCHEMICAL ENGINEERING JOURNAL, vol. 135, 2018, pages 11 - 21, XP085400608, DOI: 10.1016/j.bej.2017.11.005
BIOCHEMICAL ENGINEERING JOURNAL, vol. 120, 2017, pages 49 - 62
See also references of EP 3907278A4
ADLERZ K., TREMPEL M., WANG D., KIRIAN R.D., ROWLEY J.A., AHSAN T.: "Comparison of msc-evs manufatured in 2D versus scalable 3D bioreactor systems", CYTOTHERAPY, ISIS MEDICAL MEDIA, OXFORD,, GB, vol. 21, no. 5, 1 May 2019 (2019-05-01), GB , pages S58, XP055859057, ISSN: 1465-3249, DOI: 10.1016/j.jcyt.2019.03.434
DE SOURE, A. M. ET AL.: "Scalable microcarrier- based manufacturing of mesenchymal stem/stromal cells", JOURNAL OF BIOTECHNOLOGY, vol. 236, 2016, pages 88 - 109, XP029729470, DOI: 10.1016/j.jbiotec.2016.08.007
CHEN, A. K. L. ET AL.: "Application of human mesenchymal and pluripotent stem cell microcarrier cultures in cellular therapy: Achievements and future direction", BIOTECHNOLOGY ADVANCES, vol. 31, 2013, pages 1032 - 1046, XP055113967, DOI: 10.1016/j.biotechadv.2013.03.006
LAWSON, T. ET AL.: "Process development for expansion of human mesenchymal stromal cells in a 50 L single-use stirred tank bioreactor", BIOCHEMICAL ENGINEERING JOURNAL, vol. 120, 2017, pages 49 - 62, XP029932798, DOI: 10.1016/j.bej.2016.11.020
RAFIQ, Q. A. ET AL.: "Qualitative and quantitative demonstration of bead-to-bead transfer with bone marrow-derived human mesenchymal stem cells on microcarriers: Utilising the phenomenon to improve culture performance", BIOCHEMICAL ENGINEERING JOURNAL, vol. 135, 2018, pages 11 - 21, XP085400608, DOI: 10.1016/j.bej.2017.11.005
BIOCHEMICAL ENGINEERING JOURNAL, vol. 120, 2017, pages 49 - 62
See also references of EP 3907278A4
Attorney, Agent or Firm:
MIYOSHI Hidekazu et al. (JP)
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