Title:
METHOD FOR MEASURING MEMBRANE POTENTIAL/MEMBRANE CURRENT OF CELL
Document Type and Number:
WIPO Patent Application WO/2018/199334
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a method for accurately measuring and controlling intracellular potential by a simple method that is less invasive to the cell and does not require a skilled technique. The present invention makes it possible to provide an intracellular recording electrode inside the cytoplasm by introducing conductive nanoparticles into a cell cultured on a conductive plate electrode, attracting the conductive nanoparticles inside the cell to the side of the cell adhered to the conductive plate electrode, and causing the conductive nanoparticles to pass through the cell membrane. Measuring the current or voltage between the intracellular recording electrode and an extracellular electrode in extracellular solution makes it possible to measure the intracellular potential. In addition, applying a current from one of the electrodes or applying a voltage makes it possible to control the intracellular potential and to measure the activity of the ion channels using a membrane potential fixation method. Similarly, using a magnetic electrode adhered to the cell surface of a target cell into which conductive nanoparticles have been introduced beforehand to attract the conductive nanoparticles in the cell to the side of the cell adhered to the electrode and cause the conductive nanoparticles to pass through the cell membrane to make contact with the magnetic electrode, makes it possible to provide an intracellular recording electrode inside the cytoplasm. Alternatively, adhering conductive nanoparticles adsorbed to the surface of a magnetic electrode to the upper side of the target cell and causing the conductive nanoparticles to pass through the cell membrane by attracting the conductive particles to an iron plate provided on the lower side of the cell thereby forms an intracellular recording electrode.
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Inventors:
SAITO MITSUYOSHI (JP)
Application Number:
PCT/JP2018/017334
Publication Date:
November 01, 2018
Filing Date:
April 27, 2018
Export Citation:
Assignee:
ION CHAT RES CORPORATE (JP)
SAITO MITSUYOSHI (JP)
SAITO MITSUYOSHI (JP)
International Classes:
C12Q1/02; C12M1/34; G01N27/00; C12N15/09
Domestic Patent References:
| WO2012043820A1 | 2012-04-05 | |||
| WO2013061849A1 | 2013-05-02 | |||
| WO2014098182A1 | 2014-06-26 | |||
| WO2014192312A1 | 2014-12-04 |
Foreign References:
| JP2012503492A | 2012-02-09 | |||
| JP2005505761A | 2005-02-24 | |||
| JP2006217866A | 2006-08-24 |
Other References:
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KIM, YONG-HEE ET AL.: "Multi-Electrode Arrays Modified with Bimetallic Nanoparticles; Electrical Performance and Neural Signal Recording", PROCEEDINGS OF THE 15TH IEEE INTERNATIONAL CONFERENCE ON NANOTECHNOLOGY, 2015, pages 238 - 241, XP032853961
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T. TOMITA., BIO. SOCI. JAPAN GENERAL INCORPORATED ASSOCIATION, vol. 34, no. 6, 1994, pages 6 - 11
MOSCHO ET AL., PNAS, vol. 93, 1996, pages 11443 - 11447
SCHLESINGER SAITO, CELL DEATH AND DIFFERENTIATION, vol. 13, 2006, pages 1403 - 1408
AIMON ET AL., PLOS ONE, vol. 6, no. 10, 2011, pages e25529
WANG ET AL., J. BIOL. CHEM., vol. 284, no. 9, 2009, pages 5685 - 5696
KAMI, DAISUKE: "Application of Magnetic Nanoparticles to Gene Delivery", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 12, no. 6, 7 June 2011 (2011-06-07), pages 3705 - 3722, XP055528386
GRAMOWSKI, ALEXANDRA ET AL.: "Nanoparticles Induce Changes of the Electrical Activity of Neuronal Networks on Microelectrode Array Neurochips", ENVIRON HEALTH PERSPECT, vol. 118, no. 10, October 2010 (2010-10-01), pages 1363 - 1369, XP055528389
KIM, YONG-HEE ET AL.: "Multi-Electrode Arrays Modified with Bimetallic Nanoparticles; Electrical Performance and Neural Signal Recording", PROCEEDINGS OF THE 15TH IEEE INTERNATIONAL CONFERENCE ON NANOTECHNOLOGY, 2015, pages 238 - 241, XP032853961
ANNA FENDYUR ET AL., FRONTIERS IN NEUROENGINEERING, vol. 4, December 2011 (2011-12-01), pages 1 - 14
RAPHAEL LEVY ET AL., NANOREVIEWS, 2010, pages 1
MICHA E. SPIRA ET AL., NATURE, vol. 8, February 2013 (2013-02-01), pages 83 - 94
ROSABELOTI: "Effect of cp Ti Surface Roughness on Human Bone Marrow Cell Attachment, Proliferation, and Differentiation", BRAZ DENT J, vol. 14, no. 1, 2003, pages 16 - 21
YAMADA ET AL., WIRES NANOMED NANOBIOTECHNOL, vol. 7, 2015, pages 428 - 445
00 OTELAJAD.-H. HAT. LYH. ZHANGRD ROBINSON: "Highly Conductive Cu2-xS Nanoparticle Films, which have been conventionally used for staining of biological imaging. Through Room Temperature Processing, and an Order of Magnification Enhancement of Conductivity via Electrophoretic Deposition", ACS APPLIED MATERIALS AND INTERFACES, vol. 6, 2014, pages 18911 - 18920
WALEV ET AL., PNAS, vol. 98, no. 6, 2001, pages 3185 - 3190
T. TOMITA., BIO. SOCI. JAPAN GENERAL INCORPORATED ASSOCIATION, vol. 34, no. 6, 1994, pages 6 - 11
MOSCHO ET AL., PNAS, vol. 93, 1996, pages 11443 - 11447
SCHLESINGER SAITO, CELL DEATH AND DIFFERENTIATION, vol. 13, 2006, pages 1403 - 1408
AIMON ET AL., PLOS ONE, vol. 6, no. 10, 2011, pages e25529
WANG ET AL., J. BIOL. CHEM., vol. 284, no. 9, 2009, pages 5685 - 5696
Attorney, Agent or Firm:
SAEKI, Takuro et al. (JP)
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