Title:
METHOD FOR MEASURING PHYSIOLOGICALLY ACTIVE SUBSTANCE OF BIOLOGICAL ORIGIN
Document Type and Number:
WIPO Patent Application WO/2012/029171
Kind Code:
A1
Abstract:
A technique is provided in which measurement precision can be increased or measurement time reduced in measurement of a physiologically active substance of biological origin, such as an endotoxin or β-D-glucan, in hyaluronic acid. A hyaluronic acid preparation and water for injection are heated to a temperature of 30−40°C, and the hyaluronic acid preparation is then diluted by a factor of 10−200 with the water for injection. The diluted specimen thus obtained is further brought to 36−40°C and mixed with AL reagent, and the concentration of an endotoxin or β-D-glucan is measured using a laser diffraction particle analyzer.
Inventors:
HIRONO TAISUKE (JP)
ASANO TAKAHARU (JP)
ASANO TAKAHARU (JP)
Application Number:
PCT/JP2010/065135
Publication Date:
March 08, 2012
Filing Date:
September 03, 2010
Export Citation:
Assignee:
KOWA CO (JP)
HIRONO TAISUKE (JP)
ASANO TAKAHARU (JP)
HIRONO TAISUKE (JP)
ASANO TAKAHARU (JP)
International Classes:
G01N33/579
Foreign References:
| JP2005308574A | 2005-11-04 | |||
| JP2007064895A | 2007-03-15 | |||
| JP2004093536A | 2004-03-25 | |||
| JP2006194606A | 2006-07-27 |
Attorney, Agent or Firm:
KAWAGUCHI, Yoshiyuki et al. (JP)
Yoshiyuki Kawaguchi (JP)
Yoshiyuki Kawaguchi (JP)
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