METHOD FOR MONITORING DIFFERENTIATION TO MYOCARDIAL CELLS

Title:

METHOD FOR MONITORING DIFFERENTIATION TO MYOCARDIAL CELLS

Document Type and Number:

WIPO Patent Application WO/2015/162810

Kind Code:

A1

Abstract:

In the present invention, a method for monitoring differentiation to myocardial cells includes maintaining in a viable state cells which are from a step (S1) and to which were introduced reporter genes of light-emitting proteins that have been constructed such that the amount of light emitted varies with expression of myocardial differentiation marker genes. The method also includes the following: a step (S2) for obtaining a light emission image as a still image by capturing, in a light shielded state, the light emitted from the cells; a step (S3) for obtaining sequential images by illuminating the cells; and a step (S6) for correlating the biological data obtained from the sequential images and the still image.

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Inventors:

HAYASHI TARO (JP)
SAKANE ISAO (JP)
OHASHI YOKO (JP)

Application Number:

PCT/JP2014/076193

Publication Date:

October 29, 2015

Filing Date:

September 30, 2014

Export Citation:

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Assignee:

OLYMPUS CORP (JP)

International Classes:

C12Q1/02; C12N5/10; C12N15/09

Domestic Patent References:

WO2011118655A1	2011-09-29
WO2013137491A1	2013-09-19

Foreign References:

JP2014033617A	2014-02-24
JP2013192468A	2013-09-30
JP2013195127A	2013-09-30
JP2013053854A	2013-03-21
JP2012194168A	2012-10-11

Other References:

TARO HAYASHI ET AL.: "Hakko Imaging ni yoru Shinkin Bunka Marker Idenshi Hatsugen no Kashika", REGENERATIVE MEDICINE, vol. 13, 27 January 2014 (2014-01-27), pages 323