Title:
METHOD FOR OBTAINING STERILIZED CYST NEMATODE
Document Type and Number:
WIPO Patent Application WO/2019/160075
Kind Code:
A1
Abstract:
The present invention addresses the problem of sterilizing cyst nematodes, establishing an in-vitro mass propagation system for cyst nematodes, and providing sterilized cyst nematodes that allow evaluation tests such as an egg hatch assay to be stably conducted. The present invention relates to a method for obtaining sterilized cyst nematodes, the method including: culturing cyst nematodes, which are to be sterilized, on a sand medium in which a host plant is planted; and irrigating the sand medium with a drug. The drug used in the irrigation includes a germicidal at a concentration that does not affect the growth of the nematodes. The present invention also relates to sterilized cyst nematodes obtained by said method.
Inventors:
HAYASHI KOTOMI (JP)
MIYASHIRO HIROKO (JP)
WANIBUCHI KYOKO (JP)
ONO TEIKO (JP)
TABAYASHI NORIKO (JP)
MIYASHIRO HIROKO (JP)
WANIBUCHI KYOKO (JP)
ONO TEIKO (JP)
TABAYASHI NORIKO (JP)
Application Number:
PCT/JP2019/005512
Publication Date:
August 22, 2019
Filing Date:
February 15, 2019
Export Citation:
Assignee:
HOKUSAN KK (JP)
International Classes:
C12N5/00; A01K67/033; G01N33/15
Foreign References:
| JP2016520522A | 2016-07-14 | |||
| JPH10510146A | 1998-10-06 |
Other References:
HANSEN E. ET AL.: "Differential expression patterns of the wound-inducible transgene wun1-uidA in potato roots following infection with either cyst or root knot nematodes", PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, vol. 48, no. 3, March 1996 (1996-03-01), pages 161 - 170, XP055634910
KUSHå·¥DA, ATSUHIKO ET AL: "Axenic Culture of Heterodera glycines on Milk-Vetch Roots Genetically Transformed by Agrobacterium rhizogenes", ANNUAL REPORT OF THE SOCIETY OF PLANT PROTECTION OF NORTH JAPAN, vol. 2002, no. 53, 2002, pages 297 - 300, XP055634919
FOOT M. A.: "Laboratory rearing of potato cyst nematode; a method suitable for pathotyping and biological studies", NEW ZEALAND JOURNAL OF ZOOLOGY, vol. 4, no. 2, June 1977 (1977-06-01), pages 183 - 186, XP055634912
TRIFONOVA Z. ET AL.: "Fungal parasitism of the cysts and eggs of the Globodera rostochiensis", JOURNAL OF AGRICULTURAL SCIENCES, vol. 48, no. 1, 2003, pages 103 - 110, XP055634913
KUSHå·¥DA, ATSUHIKO ET AL: "Axenic Culture of Heterodera glycines on Milk-Vetch Roots Genetically Transformed by Agrobacterium rhizogenes", ANNUAL REPORT OF THE SOCIETY OF PLANT PROTECTION OF NORTH JAPAN, vol. 2002, no. 53, 2002, pages 297 - 300, XP055634919
FOOT M. A.: "Laboratory rearing of potato cyst nematode; a method suitable for pathotyping and biological studies", NEW ZEALAND JOURNAL OF ZOOLOGY, vol. 4, no. 2, June 1977 (1977-06-01), pages 183 - 186, XP055634912
TRIFONOVA Z. ET AL.: "Fungal parasitism of the cysts and eggs of the Globodera rostochiensis", JOURNAL OF AGRICULTURAL SCIENCES, vol. 48, no. 1, 2003, pages 103 - 110, XP055634913
Attorney, Agent or Firm:
KUZUWA, Kiyoshi (JP)
Download PDF: