Title:
METHOD OF PIERCING MEMBRANE AND APPARATUS THEREFOR
Document Type and Number:
WIPO Patent Application WO/2001/019953
Kind Code:
A1
Abstract:
A method which comprises bringing a membrane denaturing material, which undergoes a membrane denaturation reaction induced by a stimulus, into contact with at least a part of a membrane (10) or close thereto; thereby stimulating the membrane denaturing material so as to denature the membrane (10); and piercing the membrane (10) by using a membrane breaking member (1); wherein the stimulus is imparted via the membrane breaking member (10). Thus, the effect of the membrane breaking agent on the substance, in which the stimulus is transmembranely injected, can be prevented as far as possible. It is also possible to favorably stimulate a specific site, though the apparatus has a simple constitution.
Inventors:
SAITO TAKASHI (JP)
Application Number:
PCT/JP2000/006045
Publication Date:
March 22, 2001
Filing Date:
September 06, 2000
Export Citation:
Assignee:
CT FOR ADVANCED SCIENCE & TECH (JP)
SAITO TAKASHI (JP)
SAITO TAKASHI (JP)
International Classes:
C12M3/00; C12N15/87; C12N15/89; (IPC1-7): C12M1/00; C12N15/00
Foreign References:
| JPH08322548A | 1996-12-10 |
Other References:
TAKASHI SAITO ET AL.: "Kouzoukan sayou wo riyou shita micro injection", SEITAI SEIRI KOUGAKU SYMPOSIUM RONBUNSHU, vol. 14, 1999, pages 77 - 80, XP002934210
SAITO T. ET AL.: "Light dose and time dependency of photodynamic cell membrane damage", PHOTOCHEM. PHOTOBIOL., vol. 68, no. 5, 1998, pages 745 - 748, XP002934211
ATSUSHI KITAYAMA ET AL.: "Denki senkouhou ni yoru bacteria e no DNA no dounyu", CHEMICAL ENGINEERING, vol. 35, no. 12, 1990, pages 944 - 948, 978, XP002934212
NOBUO KIMURA ET AL.: "Laser shiki self processor no kaihatsu", O PLUS E, vol. 113, 1989, pages 100 - 103, XP002934213
YOSHIHIRO KASUYA ET AL.: "Laser saibou shujutsu ni yoru idenshi inyu", LASER KENKYU, vol. 15, no. 8, 1987, pages 624 - 635, XP002934214
See also references of EP 1211307A4
SAITO T. ET AL.: "Light dose and time dependency of photodynamic cell membrane damage", PHOTOCHEM. PHOTOBIOL., vol. 68, no. 5, 1998, pages 745 - 748, XP002934211
ATSUSHI KITAYAMA ET AL.: "Denki senkouhou ni yoru bacteria e no DNA no dounyu", CHEMICAL ENGINEERING, vol. 35, no. 12, 1990, pages 944 - 948, 978, XP002934212
NOBUO KIMURA ET AL.: "Laser shiki self processor no kaihatsu", O PLUS E, vol. 113, 1989, pages 100 - 103, XP002934213
YOSHIHIRO KASUYA ET AL.: "Laser saibou shujutsu ni yoru idenshi inyu", LASER KENKYU, vol. 15, no. 8, 1987, pages 624 - 635, XP002934214
See also references of EP 1211307A4
Attorney, Agent or Firm:
Inaba, Shigeru (Inaba & Inami Hanabishi Imas Hirakawacho
Building 4th Floor 3-11 Hirakawacho 2-chome Chiyoda-ku, Tokyo, JP)
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