Title:
METHOD FOR PREPARING ACYLATED ANTHOCYANINS OF PAEONIDIN AND MALVIDIN
Document Type and Number:
WIPO Patent Application WO/2022/052393
Kind Code:
A1
Abstract:
A method for separating and preparing paeonidin-3-O-(6-p-coumaroyl) glucoside and malvidin-3-O-(6-p-coumaroyl) glucoside. Two high-purity acylated paeonidin and malvidin anthocyanin monomers are purified and separated from grapes by means of steps of abstraction, macroporous resin purification, extraction, preparative liquid phase chromatography and high speed countercurrent chromatography, etc. By means of said method, at least 60 mg of paeonidin-3-O-(6-p-coumaroyl) glucoside and at least 200 mg of malvidin-3-O-(6-p-coumaroyl) glucoside can be obtained from 10 kg of grape skin, and each purity can reach 98% or more. The present method has the advantages of simple operation, large processing volume, good repeatability, etc. and provides a new idea for the development and utilization of grape resources.
Inventors:
CHEN WEI (CN)
XU YANG (CN)
XIE JIAHONG (CN)
CUI HAOXIN (CN)
XU YANG (CN)
XIE JIAHONG (CN)
CUI HAOXIN (CN)
Application Number:
PCT/CN2021/070636
Publication Date:
March 17, 2022
Filing Date:
January 07, 2021
Export Citation:
Assignee:
UNIV ZHEJIANG (CN)
International Classes:
C07H17/065; C07H1/08
Foreign References:
| CN109200045A | 2019-01-15 |
Other References:
ZHAO XU; ZHANG SHAN-SHAN; ZHANG XIN-KE; HE FEI; DUAN CHANG-QING: "An effective method for the semi-preparative isolation of high-purity anthocyanin monomers from grape pomace", FOOD CHEMISTRY, ELSEVIER LTD., NL, vol. 310, 7 November 2019 (2019-11-07), NL , XP085974911, ISSN: 0308-8146, DOI: 10.1016/j.foodchem.2019.125830
LI YU-WAN, LIU QING-QING; XU ZHEN-ZHEN: "Research Advancements of Separation and Purification of Anthocyanins by High Speed Counter-Current Chromatography", FOOD AND NUTRITION IN CHINA, no. 02, 28 February 2017 (2017-02-28), pages 39 - 43, XP055911460, ISSN: 1006-9577
HONGKUN XUE, JIAQI TAN, CHAI LIU, CHENGHAI LIU: "Isolation, Purification, Structure Identification and Anti-tumor Activity of Anthocyanin from Kyoho Grape Skins", FOOD SCIENCE, BEIJING FOOD SCIENCE RESEARCH INSTITUTE, CN, vol. 41, no. 5, 1 January 2020 (2020-01-01), CN , pages 5 - 39, XP055911466, ISSN: 1002-6630
QIU FAN, LUO JIANGUANG, YAO SHUN, MA LI, KONG LINGYI: "Preparative isolation and purification of anthocyanins from purple sweet potato by high-speed counter-current chromatography", JOURNAL OF SEPARATION SCIENCE, WILEY, DE, vol. 32, no. 12, 1 June 2009 (2009-06-01), DE , pages 2146 - 2151, XP055911475, ISSN: 1615-9306, DOI: 10.1002/jssc.200900038
LI YU-WAN, LIU QING-QING; XU ZHEN-ZHEN: "Research Advancements of Separation and Purification of Anthocyanins by High Speed Counter-Current Chromatography", FOOD AND NUTRITION IN CHINA, no. 02, 28 February 2017 (2017-02-28), pages 39 - 43, XP055911460, ISSN: 1006-9577
HONGKUN XUE, JIAQI TAN, CHAI LIU, CHENGHAI LIU: "Isolation, Purification, Structure Identification and Anti-tumor Activity of Anthocyanin from Kyoho Grape Skins", FOOD SCIENCE, BEIJING FOOD SCIENCE RESEARCH INSTITUTE, CN, vol. 41, no. 5, 1 January 2020 (2020-01-01), CN , pages 5 - 39, XP055911466, ISSN: 1002-6630
QIU FAN, LUO JIANGUANG, YAO SHUN, MA LI, KONG LINGYI: "Preparative isolation and purification of anthocyanins from purple sweet potato by high-speed counter-current chromatography", JOURNAL OF SEPARATION SCIENCE, WILEY, DE, vol. 32, no. 12, 1 June 2009 (2009-06-01), DE , pages 2146 - 2151, XP055911475, ISSN: 1615-9306, DOI: 10.1002/jssc.200900038
Attorney, Agent or Firm:
HANGZHOU QIUSHI PATENT OFFICE CO., LTD. (CN)
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