Title:
METHOD FOR PREPARING CELL CULTURES FROM BIOLOGICAL SPECIMENS FOR CHEMOTHERAPEUTIC AND OTHER ASSAYS
Document Type and Number:
WIPO Patent Application WO2004015065
Kind Code:
A3
Abstract:
An improved method for preparing a cell culture is disclosed. The method includes culturing a multicellular tissue explant in the presence of growth medium that is substantially free of enzymes capable of digesting the explant and, subsequently, removing the explant at a predetermined timed.
Inventors:
KORNBLITH PAUL L (US)
GIMIGLIANO SHERI (US)
GIMIGLIANO SHERI (US)
Application Number:
PCT/US2003/023888
Publication Date:
October 05, 2006
Filing Date:
July 30, 2003
Export Citation:
Assignee:
PRECISION THERAPEUTICS INC (US)
International Classes:
C12N5/00; C12N5/09; G01N33/50
Other References:
FRESHNEY: "Culture of Animal Cells, 3rd Edition", 1994, WILEY-LISS, INC., NY, article "Chapter 9. Disaggregation of Tissue and Primary Culture", pages: 148 - 156, XP008074308
FRESHNEY: "Culture of Animal Cells, 3rd Edition", 1994, WILEY-LISS, INC., NY, article "Chapter 10, Culture of Tumor Tissue", pages: 153 - 156, XP008085114, .
FRESHNEY: "Culture of Animal Cells, 3rd Edition", 1994, WILEY-LISS, INC., NY, article "Chapter 10, Culture of Tumor Tissue", pages: 153 - 156, XP008085114, .
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