Title:
METHOD FOR PREPARING COBALT−PROTEIN COMPLEX
Document Type and Number:
WIPO Patent Application WO/2003/031322
Kind Code:
A1
Abstract:
A method for preparing a cobalt−apoferritin complex which comprises a first step (St1 in Fig.2) of mixing a HEPES buffer solution, an apoferritin solution and a Co2&plus ion solution (for example, cobalt nitrate solution) in this order, to prepare a reaction solution, and a second step (St2) of adding an oxidizing agent (for example, H2O2) to the reaction solution, to thereby introduce cobalt hydroxide (CoO(OH)) into the holding sector (4) of apoferritin (1) and form a cobalt−apoferritin complex. The above operations for forming a cobalt−apoferritin complex are carried out while agitating with a stirrer at room temperature. The method allows the preparation of a cobalt−apoferritin complex including fine cobalt particles having a uniform particle diameter.
Inventors:
YAMASHITA ICHIRO (JP)
Application Number:
PCT/JP2002/010127
Publication Date:
April 17, 2003
Filing Date:
September 27, 2002
Export Citation:
Assignee:
MATSUSHITA ELECTRIC IND CO LTD (JP)
YAMASHITA ICHIRO (JP)
YAMASHITA ICHIRO (JP)
International Classes:
B82B3/00; C07K1/02; C07K14/47; H01L21/8247; H01L27/115; H01L29/06; C07K14/435; H01L29/788; H01L29/792; H01L21/28; (IPC1-7): B82B3/00; H01L51/00; H01L21/8247; H01L29/788; H01L27/115; C07K14/47; C07K1/02
Domestic Patent References:
| WO1991002704A1 | 1991-03-07 |
Other References:
DOUGLAS T. AND STARK V.T.: "Nanophase cobalt oxyhydroxide mineral synthesized within the protein cage of ferritin", INORGANIC CHEMISTRY, vol. 39, no. 8, 17 April 2000 (2000-04-17), pages 1828 - 1830, XP002961044
YANG X. AND CHASTEEN N.D.: "Ferroxidase activity of ferritin: effects of pH, buffer and Fe(II) and Fe(III) concentrations on Fe(II) autoxidation and ferroxidation", BIOCHEMICAL JOURNAL, vol. 338, no. 3, 15 March 1999 (1999-03-15), pages 615 - 618, XP002961045
MELDRUM F.C. ET AL.: "Magnetoferritin: in vitro synthesis of a novel magnetic protein", SCIENCE, vol. 257, 24 July 1992 (1992-07-24), pages 522 - 523, XP000676756
YANG X. AND CHASTEEN N.D.: "Ferroxidase activity of ferritin: effects of pH, buffer and Fe(II) and Fe(III) concentrations on Fe(II) autoxidation and ferroxidation", BIOCHEMICAL JOURNAL, vol. 338, no. 3, 15 March 1999 (1999-03-15), pages 615 - 618, XP002961045
MELDRUM F.C. ET AL.: "Magnetoferritin: in vitro synthesis of a novel magnetic protein", SCIENCE, vol. 257, 24 July 1992 (1992-07-24), pages 522 - 523, XP000676756
Attorney, Agent or Firm:
Maeda, Hiroshi (4-8 Utsubohonmachi 1-chome, Nishi-k, Osaka-shi Osaka, JP)
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