Title:
METHOD FOR PREPARING IMMOBILIZED ENZYME HAVING HIGH STABILITY
Document Type and Number:
WIPO Patent Application WO/2023/186186
Kind Code:
A1
Abstract:
A method for preparing an immobilized enzyme having stability. The method comprises: adding amino resin LXTE-700S into a crude enzyme solution for filtration, and then adding a boric acid buffer solution and pyridoxal phosphate, and carrying out an oscillation reaction; and finally, adding a long-range cross-linking agent and a short-range cross-linking agent, carrying out an oscillation reaction and then vacuum filtration to obtain the immobilized enzyme having high stability. The epoxy-based cross-linking agent is a mixture of a polyethylene glycol diglycidyl ether long-range cross-linking agent and a glycerol triglycidyl ether short-range cross-linking agent. The method for preparing the immobilized enzyme involves simple operations, and allows the storage and service life of the immobilized enzyme to be markedly prolonged.
Inventors:
RAO JINGWEI (CN)
WANG HAIYONG (CN)
YUE YONGLI (CN)
SHEN YANYANG (CN)
LU FEI (CN)
LIN LI (CN)
YU BING (CN)
WANG HAIYONG (CN)
YUE YONGLI (CN)
SHEN YANYANG (CN)
LU FEI (CN)
LIN LI (CN)
YU BING (CN)
Application Number:
PCT/CN2023/095548
Publication Date:
October 05, 2023
Filing Date:
May 22, 2023
Export Citation:
Assignee:
ANQING ROUNDCARE PHARMACEUTICAL CO LTD (CN)
International Classes:
C12N9/10; C12N11/091; C12N11/089
Domestic Patent References:
| WO2021128086A1 | 2021-07-01 |
Foreign References:
| CN114657170A | 2022-06-24 | |||
| CN113817699A | 2021-12-21 | |||
| CN110964710A | 2020-04-07 |
Other References:
MINGHUA QIAN, ZHANG JIFU, ZHANG YUN, SUN AIJUN, YU RONGMIN: "Immobilization of lipase on macroporous resin by adsorption-crosslinking method ", JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY, CN, vol. 40, no. 2, 30 January 2019 (2019-01-30), CN , pages 103 - 110, XP093095373, ISSN: 1001-411X, DOI: 10.7671/j.issn.1001-411X.201804031
Attorney, Agent or Firm:
JIANGSU SUNDY LAW FIRM (CN)
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