Title:
METHOD FOR PRODUCING DNA PROBE AND METHOD FOR ANALYZING GENOMIC DNA USING THE DNA PROBE
Document Type and Number:
WIPO Patent Application WO/2018/003727
Kind Code:
A1
Abstract:
This invention provides a DNA probe that is applicable to a DNA library prepared in a simple manner with excellent reproducibility. Such DNA probe is produced by a method comprising steps of performing a nucleic acid amplification reaction in a reaction solution containing genomic DNA and a random primer at a high concentration, so as to obtain a DNA fragments with the use of the genomic DNA as a template; determining the nucleotide sequence of the resulting DNA fragments; and, on the basis of the nucleotide sequence of the DNA fragments obtained in the step above, designing a DNA probe used for detecting a DNA fragment.
Inventors:
ENOKI HIROYUKI (JP)
TAKEUCHI YOSHIE (JP)
INAMORI MINORU (JP)
TAKEUCHI YOSHIE (JP)
INAMORI MINORU (JP)
Application Number:
PCT/JP2017/023343
Publication Date:
January 04, 2018
Filing Date:
June 26, 2017
Export Citation:
Assignee:
TOYOTA MOTOR CO LTD (JP)
International Classes:
C12Q1/68; C12N15/10
Domestic Patent References:
| WO2008101701A2 | 2008-08-28 |
Foreign References:
| EP2514820A1 | 2012-10-24 | |||
| EP1721970A1 | 2006-11-15 | |||
| JP5389638B2 | 2014-01-15 | |||
| JP2003079375A | 2003-03-18 | |||
| JP3972106B2 | 2007-09-05 | |||
| JP5799484B2 | 2015-10-28 | |||
| JP2014204730A | 2014-10-30 |
Other References:
DNA RESEARCH, vol. 21, 2014, pages 555 - 567
BREEDING SCIENCE, vol. 64, 2014, pages 291 - 299
BREEDING SCIENCE, vol. 64, 2014, pages 291 - 299
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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