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Title:
METHOD FOR PRODUCING ETHANOL
Document Type and Number:
WIPO Patent Application WO/2017/134975
Kind Code:
A1
Abstract:
The method for producing ethanol according to the present invention comprises (A) a step for mixing a pretreated lignocellulosic biomass with an enzyme to saccharify the lignocellulosic biomass, and (B) a step for inoculating, with a yeast, and fermenting a saccharification liquid and saccharification residue obtained in step (A), wherein: the yeast is Saccharomyces cerevisiae; and the method comprises, before step (A), or after step (A) but before step (B), (M) a step for determining the concentration of yeast cells in step (B) so as to equal to a preset reference value depending on the concentration of furfural in a liquid containing the pretreated lignocellulosic biomass before step (A) or the saccharification liquid obtained in step (A), wherein the preset reference value is the concentration of yeast cells which is higher than or equal to the critical value of the concentration of yeast cells at which fermentation is not inhibited depending on the concentration of furfural, and the yeast is inoculated in step (B) such that the concentration of yeast cells determined in step (M) is attained.

Inventors:
KIUCHI TAKAFUMI (JP)
MORITA KENTARO (JP)
KOGA TSUKASA (JP)
OGAWA KENICHI (JP)
MYOGA NATSUKI (JP)
YASUTANI NORIKO (JP)
ONISHI TORU (JP)
Application Number:
PCT/JP2017/000099
Publication Date:
August 10, 2017
Filing Date:
January 05, 2017
Export Citation:
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Assignee:
NIPPON STEEL & SUMIKIN ENG CO (JP)
TOYOTA MOTOR CO LTD (JP)
International Classes:
C12P7/06; C12N1/16; C12P1/00; C12P7/10
Domestic Patent References:
WO2009110374A12009-09-11
Foreign References:
JP2012235729A2012-12-06
JP2014176351A2014-09-25
JP2015107088A2015-06-11
JP2011167096A2011-09-01
Other References:
OLIVA JM. ET AL.: "Effects of acetic acid, furfural and catechol combinationson ethanol fermentation of Kluyveromyces marxianus", PROCESS BIOCHEMISTRY, vol. 41, no. 5, 2006, pages 1223 - 1228, XP027984141
TOFIGHI A. ET AL.: "Inhibitory Effect of High Concentrations of Furfural onIndustrial Strain of Saccharomyces cerevisiae", INT. J. ENVIRON. RES., vol. 4, no. 1, 2010, pages 137 - 142, XP055404256
TAHERZADEH MJ. ET AL.: "Inhibition Effects of Furfural on Aerobic Batch Cultivation of Saccharomyces cerevisiae Growing on Ethanol and/or Acetic Acid", J. BIOSCI. BIOENG., vol. 90, no. 4, 2000, pages 374 - 380, XP055404259
Attorney, Agent or Firm:
SHIGA Masatake et al. (JP)
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