Title:
METHOD FOR PRODUCING EXTRACELLULAR PARTICLES
Document Type and Number:
WIPO Patent Application WO/2023/026830
Kind Code:
A1
Abstract:
[Problem] To provide a method for producing extracellular particles usable for a vaccine for a swine infectious disease. [Solution] A method for producing extracellular particles that comprises: infecting swine cells, which can be infected with PRRS virus, with the virus at a moi (multiple of infection) concentration of 0.01-1.0 and, at the same time, adding interferon (INF) thereto; removing lysed cells by washing; and adding a culture solution to the unlysed cells remaining in the incubator, said unlysed cells containing a large amount of persistently virus-infected cells. After repeating the steps for washing and for adding the culture solution a preset number of times, extracellular particles are separated and purified from the remaining persistently virus-infected cells. Cells have individual differences (individuality) and, therefore, different sensitivities to interferon (IFN). Accordingly, not all cells become persistently infected even under the same experimental conditions. Experiment 2 in FIG. 2 shows an example in which the cells did not become persistently virus-infected cells even under the same experimental conditions. Therefore, it is necessary to analyze the proteins expressed by the cells after infection with the virus, and to select and clone the most promising persistently infected cells for vaccine production.
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Inventors:
SAKUMA SADATOSHI (JP)
Application Number:
PCT/JP2022/030152
Publication Date:
March 02, 2023
Filing Date:
August 05, 2022
Export Citation:
Assignee:
VAXOSOME LLC (JP)
International Classes:
C12N5/10; C12N1/02; C12N5/071; C12N7/00; A61K39/12; A61P11/00; A61P15/00
Foreign References:
| JP2011041534A | 2011-03-03 | |||
| JP2006061057A | 2006-03-09 | |||
| JP2006230397A | 2006-09-07 |
Other References:
GALLO ALESSIA, VELLA SERENA, MIELE MONICA, TIMONERI FRANCESCA, DI BELLA MARIANGELA, BOSI SILVIA, SCIVERES MARCO, CONALDI PIER GIUL: "Global profiling of viral and cellular non-coding RNAs in Epstein–Barr virus-induced lymphoblastoid cell lines and released exosome cargos", CANCER LETTERS, NEW YORK, NY, US, vol. 388, 1 March 2017 (2017-03-01), US , pages 334 - 343, XP093039238, ISSN: 0304-3835, DOI: 10.1016/j.canlet.2016.12.003
Attorney, Agent or Firm:
KOYAMA Yu (JP)
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