Title:
METHOD FOR PRODUCING FUSION PROTEIN
Document Type and Number:
WIPO Patent Application WO/2022/191253
Kind Code:
A1
Abstract:
A method for producing a fusion protein comprising BDNF and an anti-transferrin receptor antibody or a fragment thereof, the method comprising a step for culturing a transformed mammalian cell containing a gene encoding the fusion protein and a gene encoding an exogenous chaperone protein in a culture medium for protein production use to produce the fusion protein and a step for collecting the produced fusion protein, in which the gene encoding the fusion protein comprises a nucleotide sequence for a gene encoding the BDNF and a nucleotide sequence for a gene encoding the anti-transferrin receptor antibody or a fragment thereof, and the chaperone protein comprises at least one member selected from the group consisting of HSP90α, HSP90β, CDC37, HSP70, HSP40, HSP60, HSP10, HSP110 and HSP27.
Inventors:
NAGATA HIDETAKA (JP)
LIN WENLIEN (JP)
ASADA REIKO (JP)
TAKIKAWA KENJI (JP)
LIN WENLIEN (JP)
ASADA REIKO (JP)
TAKIKAWA KENJI (JP)
Application Number:
PCT/JP2022/010398
Publication Date:
September 15, 2022
Filing Date:
March 09, 2022
Export Citation:
Assignee:
SUMITOMO PHARMA CO LTD (JP)
International Classes:
C12P21/02; C07K14/47; C07K16/18; C07K19/00; C12N5/10; C12N15/12; C12N15/13; C12N15/62; C12N15/63
Domestic Patent References:
| WO2016208696A1 | 2016-12-29 | |||
| WO2018124107A1 | 2018-07-05 | |||
| WO2010010848A1 | 2010-01-28 | |||
| WO1994008012A1 | 1994-04-14 | |||
| WO2016208696A1 | 2016-12-29 | |||
| WO2018124107A1 | 2018-07-05 |
Foreign References:
| JP2007524381A | 2007-08-30 | |||
| JP2003501014A | 2003-01-14 | |||
| JP2013502424A | 2013-01-24 | |||
| JP2007524381A | 2007-08-30 |
Other References:
LEE ET AL., JOURNAL OF BIOTECHNOLOGY, vol. 143, 2009, pages 34 - 43
MOSES V. CHAO, NATURE REVIEWS NEUROSCIENCE, vol. 4, 2003, pages 299 - 309
BOLLEN E, BEHAVIOURAL BRAIN RESEARCH, 2013, pages 8 - 12
JONES ET AL., EMBO J, vol. 1, 1982, pages 549 - 553
PROC. NATL. ACAD. SCI. USA, vol. 87, 1990, pages 8060 - 8064
SAMBROOK ET AL., MOLECULAR CLONING-A LABORATORY MANUAL, 1989
J. BIOTECHNOLOGY, vol. 143, 2009, pages 34 - 43
MOSES V. CHAO, NATURE REVIEWS NEUROSCIENCE, vol. 4, 2003, pages 299 - 309
BOLLEN E, BEHAVIOURAL BRAIN RESEARCH, 2013, pages 8 - 12
JONES ET AL., EMBO J, vol. 1, 1982, pages 549 - 553
PROC. NATL. ACAD. SCI. USA, vol. 87, 1990, pages 8060 - 8064
SAMBROOK ET AL., MOLECULAR CLONING-A LABORATORY MANUAL, 1989
J. BIOTECHNOLOGY, vol. 143, 2009, pages 34 - 43
Attorney, Agent or Firm:
FUKAMI PATENT OFFICE, P.C. (JP)
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