Title:
METHOD FOR PRODUCING MULTISPECIFIC ANTIGEN-BINDING MOLECULES
Document Type and Number:
WIPO Patent Application WO/2021/201087
Kind Code:
A1
Abstract:
Multispecific antigen-binding molecule capable of binding to multiple different antigens, but do not non-specifically crosslink two or more immune cells such as T cells are provided. Methods for producing or enriching a preferred structural form of such multispecific antibody protein, and method for eliminating disulfide heterogeneity of such multispecific antibody proteins are provided. In addition, conformation-specific antibodies that specifically recognize the preferred form of multispecific antibody proteins, and use of the conformation-specific antibodies are provided.
Inventors:
CHICHILI VISHNU PRIYANKA REDDY (SG)
PANG CHAI LING (SG)
KAWA TATSUYA (JP)
FENG SHU (SG)
GAN SIOK WAN (SG)
TAKAHASHI NORIYUKI (SG)
MURAOKA MASARU (JP)
PANG CHAI LING (SG)
KAWA TATSUYA (JP)
FENG SHU (SG)
GAN SIOK WAN (SG)
TAKAHASHI NORIYUKI (SG)
MURAOKA MASARU (JP)
Application Number:
PCT/JP2021/013795
Publication Date:
October 07, 2021
Filing Date:
March 31, 2021
Export Citation:
Assignee:
CHUGAI PHARMACEUTICAL CO LTD (JP)
International Classes:
C07K16/46; C07K1/113; C07K16/28; C12N15/13
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See also references of EP 4126970A4
PORTER, N ENGL J MED, vol. 365, 2011, pages 725 - 733
DUBROT, CANCER IMMUNOL. IMMUNOTHER., vol. 28, 2010, pages 512 - 22
SCHABOWSKY, VACCINE, vol. 28, 2009, pages 512 - 22
LI, PROC NATL ACAD SCI USA., vol. 110, no. 48, 2013, pages 19501 - 6
NATURE, vol. 314, no. 6012, 18 April 1985 (1985-04-18), pages 628 - 31
INT J CANCER., vol. 41, no. 4, 15 April 1988 (1988-04-15), pages 609 - 15
PROC NATL ACAD SCI USA., vol. 83, no. 5, March 1986 (1986-03-01), pages 1453 - 7
PROC NATL ACAD SCI USA., vol. 92, no. 15, 18 July 1995 (1995-07-18), pages 7021 - 5
DRUG DISCOV TODAY., vol. 10, no. 18, 15 September 2005 (2005-09-15), pages 1237 - 44
INT J CANCER., vol. 100, no. 6, 20 August 2002 (2002-08-20), pages 690 - 7
CANCER IMMUNOL IMMUNOTHER, vol. 56, no. 10, 2007, pages 1637 - 44
CANCER IMMUNOL IMMUNOTHER., vol. 55, no. 5, 2006, pages 503 - 14
CANCER IMMUNOL IMMUNOTHER., vol. 58, no. 1, 2009, pages 95 - 109
NAT REV DRUG DISCOV., vol. 13, no. 11, November 2014 (2014-11-01), pages 799 - 801
VINAY, CELLULAR & MOLECULAR IMMUNOLOGY, vol. 8, 2011, pages 281 - 284
HOUOT, BLOOD, vol. 114, 2009, pages 3431 - 8
SHIELDS ET AL., J. BIOL. CHEM., vol. 9, no. 2, 2001, pages 6591 - 6604
PROC. NATL. ACAD. SCI. U.S.A., vol. 100, no. 11, 2003, pages 6353 - 6357
BREKKE ET AL., IMMUNOL, 1995
CHOTHIALESK, J. MOL. BIOL., vol. 196, 1987, pages 901 - 917
"Oligonucleotide Synthesis", 1984
J. P. MATHERP.E. ROBERTS: "Introduction to Cell and Tissue Culture", 1998, ACADEMIC PRESS
PORTOLANO ET AL., J. IMMUNOL., vol. 150, 1993, pages 880 - 887
JOURNAL OF IMMUNOLOGY, vol. 152, no. 11, 1994, pages 5368 - 5374
PERCZEL ET AL., PROTEIN ENGRG, vol. 4, 1991, pages 669 - 679
ST JOHN ET AL., PROC NATL ACAD SCI USA, vol. 96, 1999, pages 13029 - 13033
PRESTA ET AL., CANCER RES., vol. 57, 1997, pages 4593 - 4599
SCIENCE, vol. 240, no. 4855, 1988, pages 1038 - 1041
"Monoclonal Antibodies: A Practical Approach", 2000, OXFORD UNIVERSITY PRESS
KUNKEL, PROC. NATL. ACAD. SCI. USA, vol. 82, 1985, pages 488 - 492
ANNU REV. BIOPHYS. BIOMOL. STRUCT., vol. 35, 2006, pages 225 - 249
HUDSON ET AL., NAT MED, vol. 248, 2003, pages 129 - 134
GERNGROSS, NAT. BIOTECH., vol. 22, 2004, pages 1409 - 1414
LI ET AL., NAT. BIOTECH., vol. 24, pages 210 - 215
GRAHAM ET AL., J. GEN VIROL., vol. 36, 1977, pages 59
MATHER, BIOL. REPROD., vol. 23, 1980, pages 243 - 251
MATHER ET AL., ANNALS N.Y. ACAD. SCI., vol. 383, 1982, pages 44 - 68
URLAUB ET AL., PROC. NATL. ACAD. SCI. USA, vol. 77, 1980, pages 4216
JOURNAL OF PHARMACEUTICAL SCIENCES, vol. 97, 2008, pages 2426 - 2447
ANALYTICAL BIOCHEMISTRY, vol. 348, 2006, pages 24 - 39
SEEFELDT ET AL., PROTEIN SCI, vol. 13, 2004, pages 2639 - 2650
J. RHEUMATOL, vol. 34, 2007, pages 11
CLARKSON ET AL., NATURE, vol. 352, 1991, pages 624 - 628
RAVETCHKINET, ANNU. REV. IMMUNOL, vol. 1-3, 1991, pages 457 - 92
MACCALLUM ET AL., J. MOL. BIOL., vol. 262, 1996, pages 732 - 745
BMC RESEARCH NOTES, vol. 4, 2011, pages 281
PROC. NATL. ACAD. SCI. USA, vol. 103, no. 11, 2006, pages 4005 - 4010
CHEN ET AL., J. MOL. BIOL., vol. 293, 1999, pages 865 - 881
HOLLINGER ET AL., PROC NATL ACAD SCI USA, vol. 90, 1993, pages 6444 - 6448
PROC. NATL. ACAD. SCI. U.S.A., vol. 85, no. 16, 1988, pages 5879 - 5883
J IMMUNOL. METHODS, vol. 231, no. 1-2, 1999, pages 177 - 189
PROTEIN ENGINEERING, vol. 9, no. 3, 1996, pages 299 - 305
HUM. ANTIBOD. HYBRIDOMAS, vol. 1, no. 1, 1990, pages 47 - 54
BLOOD, vol. 109, 2007, pages 1185 - 1192
DAERON, IMMUNOL., vol. 15, 1997, pages 203 - 234
CAPEL ET AL., IMMUNOMETHODS, vol. 4, 1994, pages 25 - 34
DE HAAS ET AL., J. LAB. CLIN. MED., vol. 126, 1995, pages 330 - 41
GUYER ET AL., J. IMMUNOL., vol. 117, 1976, pages 587
KIM ET AL., J. IMMUNOL., vol. 24, 1994, pages 249
GHETIEWARD, IMMUNOL. TODAY, vol. 18, no. 12, 1997, pages 592 - 598
GHETIE ET AL., NATURE BIOTECHNOLOGY, vol. 15, no. 7, 1997, pages 637 - 640
HINTON ET AL., J. BIOL. CHEM., vol. 279, no. 8, 2004, pages 6213 - 6216
DAVIS ET AL., IMMUNOLOGICAL REVIEWS, vol. 190, 2002, pages 123 - 136
RIDGWAY ET AL., PROT ENG, vol. 9, 1996, pages 617 - 621
CARTER, J IMMUNOL METH, vol. 248, 2001, pages 7 - 15
CARTER, J IMMUNOL METHODS, vol. 248, 2001, pages 7 - 15
PROTEIN ENGINEERING DESIGN & SELECTION, vol. 23, 2010, pages 195 - 202
NAT BIOTECHNOL., vol. 32, no. 2, February 2014 (2014-02-01), pages 191 - 8
CHRISTOPH ET AL., NATURE BIOTECHNOLOGY, vol. 31, 2013, pages 753 - 758
THERIEN AGGRANT FEDEBER CM: "Interhelical hydrogen bonds in the CFTR membrane domain", NAT STRUCT BIOL, vol. 8, 2001, pages 597 - 601
PROTEIN SCIENCE, vol. 4, 1995, pages 2411 - 2423
See also references of EP 4126970A4
Attorney, Agent or Firm:
HARUNA, Masao et al. (JP)
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