Title:
METHOD FOR PRODUCING ODD-NUMBERED FATTY ACID ESTER, AND CULTURE COMPOSITION
Document Type and Number:
WIPO Patent Application WO/2020/054804
Kind Code:
A1
Abstract:
The present invention provides: a method for producing an odd-numbered fatty acid ester, the method making it possible to produce an odd-numbered fatty acid ester efficiently in Labyrinthulea; and a culture composition containing the odd-numbered fatty acid ester resulting from the method. The method for producing an odd-numbered fatty acid ester includes a step for culturing microorganisms belonging to the genus Aurantiochytrium or Schizochytrium in an amino-acid-supplemented medium. The amino-acid-supplemented medium contains a concentration of 10 mM or more per type of one or more types of amino acid selected from the group consisting of L-valine, L-isoleucine, L-threonine, L-methionine, D-methionine, and DL-methionine. The medium composition contains algal bodies of microorganisms belonging to the genus Aurantiochytrium or Schizochytrium, and an odd-numbered fatty acid ester in which an odd-numbered fatty acid produced by the microorganisms is ester-bonded. The odd-numbered fatty acid content relative to the microbial biomass is 0.04 g/g or higher, and the proportion of odd-numbered fatty acid in the total lipid is 25% or higher.
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Inventors:
KAYA KUNIMITSU (JP)
SHIRAISHI FUJIO (JP)
TAKEYAMA TOMOKIYO (JP)
SAKAMOTO SEIJI (JP)
SHIRAISHI FUJIO (JP)
TAKEYAMA TOMOKIYO (JP)
SAKAMOTO SEIJI (JP)
Application Number:
PCT/JP2019/035917
Publication Date:
March 19, 2020
Filing Date:
September 12, 2019
Export Citation:
Assignee:
REFINE HOLDINGS CO LTD (JP)
SEA ACT CO LTD (JP)
SEA ACT CO LTD (JP)
International Classes:
C12P7/64; C12N1/10
Domestic Patent References:
| WO2019126652A1 | 2019-06-27 |
Foreign References:
| JP2013192490A | 2013-09-30 | |||
| JP2011097865A | 2011-05-19 | |||
| CN101886044A | 2010-11-17 | |||
| CN101831387A | 2010-09-15 |
Other References:
SONG, XIAOJIN ET AL.: "Different Impacts of Short-Chain Fatty Acids on Saturated and Polyunsaturated Fatty Acid Biosynthesis in Aurantiochytrium sp. SD116", J. AGRIC. FOOD CHEM., vol. 61, no. 4, 16 October 2013 (2013-10-16), pages 9876 - 9881, XP055694054
ZHANG, KE ET AL.: "Regulation of the Docosapentaenoic Acid/Docosahexaenoic Acid Ratio (DPA/DHA Ratio) in Schizochytrium Limacinum B4D1", APPL. BIOCHEM. BIOTECHNOL., vol. 182, no. 1, 10 November 2016 (2016-11-10), pages 67 - 81, XP036229992
GONZALEZ-GARCIA, R. AXAYACATL ET AL.: "Microbial Propionic Acid Production, Fermentation", FERMENTATION, vol. 3, no. 2, 15 May 2017 (2017-05-15), pages 21, XP055563198
SCHREIBEROVA, OLGA ET AL.: "RP-HPLC/MS-APCI Analysis of Branched Chain TAG Prepared by Precursor-Directed Biosynthesis With Rhodococcus Erythropolis", LIPIDS, vol. 45, no. 8, August 2010 (2010-08-01), pages 743 - 756, XP055694059
PATIL, KAVITA P. ET AL.: "Improved synthesis of docosahexaenoic acid (DHA) using Schizochytrium limacinum SR21 and sustainable media", CHEMICAL ENGINEERING JOURNAL, vol. 268, 21 January 2015 (2015-01-21), pages 187 - 196, XP055694067
SHIRASAKA ET AL.: "Effect of cyanocobalamin and p-toluic acid on the fatty acid composition of Schizochytrium limacinum (Thraustochytriaceae, Labyrinthulomycota", MYCOSCIENCE, vol. 46, no. 6, December 2005 (2005-12-01), pages 358 - 363, XP019375470, DOI: 10.1007/s10267-005-0259-3
ZIRONI, ELISA ET AL.: "Determination of Vitamin B12 in Dairy Products by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry", ITALIAN JOURNAL OF FOOD SAFETY, vol. 3, no. 4, 4513, 18 December 2014 (2014-12-18), pages 254 - 255, XP055694072
ZHANG, KE ET AL.: "Regulation of the Docosapentaenoic Acid/Docosahexaenoic Acid Ratio (DPA/DHA Ratio) in Schizochytrium Limacinum B4D1", APPL. BIOCHEM. BIOTECHNOL., vol. 182, no. 1, 10 November 2016 (2016-11-10), pages 67 - 81, XP036229992
GONZALEZ-GARCIA, R. AXAYACATL ET AL.: "Microbial Propionic Acid Production, Fermentation", FERMENTATION, vol. 3, no. 2, 15 May 2017 (2017-05-15), pages 21, XP055563198
SCHREIBEROVA, OLGA ET AL.: "RP-HPLC/MS-APCI Analysis of Branched Chain TAG Prepared by Precursor-Directed Biosynthesis With Rhodococcus Erythropolis", LIPIDS, vol. 45, no. 8, August 2010 (2010-08-01), pages 743 - 756, XP055694059
PATIL, KAVITA P. ET AL.: "Improved synthesis of docosahexaenoic acid (DHA) using Schizochytrium limacinum SR21 and sustainable media", CHEMICAL ENGINEERING JOURNAL, vol. 268, 21 January 2015 (2015-01-21), pages 187 - 196, XP055694067
SHIRASAKA ET AL.: "Effect of cyanocobalamin and p-toluic acid on the fatty acid composition of Schizochytrium limacinum (Thraustochytriaceae, Labyrinthulomycota", MYCOSCIENCE, vol. 46, no. 6, December 2005 (2005-12-01), pages 358 - 363, XP019375470, DOI: 10.1007/s10267-005-0259-3
ZIRONI, ELISA ET AL.: "Determination of Vitamin B12 in Dairy Products by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry", ITALIAN JOURNAL OF FOOD SAFETY, vol. 3, no. 4, 4513, 18 December 2014 (2014-12-18), pages 254 - 255, XP055694072
Attorney, Agent or Firm:
ISONO INTERNATIONAL PATENT OFFICE, P.C. (JP)
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