Title:
METHOD FOR PRODUCING NAÏVE PLURIPOTENT STEM CELLS
Document Type and Number:
WIPO Patent Application WO/2016/148253
Kind Code:
A1
Abstract:
The problem is to produce and/or maintain naïve pluripotent stem cells capable of high expression of genes important to maintain an undifferentiated state, which could not be achieved by known methods for producing pluripotent stem cells. The problem of the invention can be solved because the invention makes it possible to produce naïve pluripotent stem cells capable of maintaining an undifferentiated state by introducing six genes (Oct3/4, Klf4, c-Myc, Sox2, Nanog, and Klf2) among the so-called reprogramming factors, inducing transient expression as a characteristic feature, and performing culturing in medium containing LIF, MEK inhibitor, GSK3 inhibitor, cAMP production promoter, TGF-β inhibitor, and PKC inhibitor.
Inventors:
OKANO HIDEYUKI (JP)
SHIOZAWA SEIJI (JP)
KISA FUMIHIKO (JP)
SHIOZAWA SEIJI (JP)
KISA FUMIHIKO (JP)
Application Number:
PCT/JP2016/058577
Publication Date:
September 22, 2016
Filing Date:
March 17, 2016
Export Citation:
Assignee:
ONO PHARMACEUTICAL CO (JP)
UNIV KEIO (JP)
UNIV KEIO (JP)
International Classes:
C12N5/10; C12N5/0735; C12N15/09
Foreign References:
| US20150037883A1 | 2015-02-05 | |||
| JP2014217344A | 2014-11-20 |
Other References:
TAKASHIMA YASUHIRO ET AL.: "Resetting Transcription Factor Control Circuitry toward Ground-State Pluripotency in Human", CELL, vol. 158, 2014, pages 1254 - 1269, XP029055525
FUMIHIKO KISA ET AL.: "Hito iPS Saibo ni Okeru Tanosei Jotai no Kaihen", REGENERATIVE MEDICINE, vol. 14, February 2015 (2015-02-01), pages 289, P-01 - 006, XP009505919
SEIJI SHIOZAWA ET AL.: "Common Marmoset ES Saibo ni Okeru Tanosei Jotai no Kaihen", REGENERATIVE MEDICINE, vol. 14, February 2015 (2015-02-01), pages 288, P-01 - 004, XP009505925
HANNA JACOB ET AL.: "Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCs", PNAS, vol. 107, no. 20, 2010, pages 9222 - 9227, XP055054545
HASSANI SEYEDEH-NAFISEH ET AL.: "Inhibition of TGFbeta Signaling Promotes Ground State Pluripotency", STEM CELL REV AND REP, vol. 10, 2014, pages 16 - 30, XP055335169
BUECKER CHRISTA ET AL.: "A Murine ESC-like State Facilitates Transgenesis and Homologous Recombination in Human Pluripotent Stem Cells", CELL STEM CELL, vol. 6, 2010, pages 535 - 546, XP055015337
See also references of EP 3272858A4
FUMIHIKO KISA ET AL.: "Hito iPS Saibo ni Okeru Tanosei Jotai no Kaihen", REGENERATIVE MEDICINE, vol. 14, February 2015 (2015-02-01), pages 289, P-01 - 006, XP009505919
SEIJI SHIOZAWA ET AL.: "Common Marmoset ES Saibo ni Okeru Tanosei Jotai no Kaihen", REGENERATIVE MEDICINE, vol. 14, February 2015 (2015-02-01), pages 288, P-01 - 004, XP009505925
HANNA JACOB ET AL.: "Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCs", PNAS, vol. 107, no. 20, 2010, pages 9222 - 9227, XP055054545
HASSANI SEYEDEH-NAFISEH ET AL.: "Inhibition of TGFbeta Signaling Promotes Ground State Pluripotency", STEM CELL REV AND REP, vol. 10, 2014, pages 16 - 30, XP055335169
BUECKER CHRISTA ET AL.: "A Murine ESC-like State Facilitates Transgenesis and Homologous Recombination in Human Pluripotent Stem Cells", CELL STEM CELL, vol. 6, 2010, pages 535 - 546, XP055015337
See also references of EP 3272858A4
Attorney, Agent or Firm:
AOKI, Atsushi et al. (JP)
Aoki 篤 (JP)
Aoki 篤 (JP)
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