Title:
METHOD FOR PRODUCING PROLIFERATING CELLS, METHOD FOR PRODUCING CELL PRODUCT, MESENCHYMAL STEM CELL POPULATION AND METHOD FOR PRODUCING SAME, CULTURE SUPERNATANT OF STEM CELLS AND METHOD FOR PRODUCING SAME, AND THERAPEUTIC AGENT
Document Type and Number:
WIPO Patent Application WO/2021/200744
Kind Code:
A1
Abstract:
A method for producing proliferating cells, said method comprising adhesion-culturing cells, which have been sown at a cell density of 0.002-2000 cells/cm2, in a proliferation culture solution in the presence of a culture substrate selected from a laminin fragment having integrin binding activity and a variant thereof and thus proliferating the cells.
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Inventors:
YANAGITA YASUTOMO (JP)
RINOIE CHUGO (JP)
ISHIDAO TAKEFUMI (JP)
MINAMI ITSUNARI (JP)
RINOIE CHUGO (JP)
ISHIDAO TAKEFUMI (JP)
MINAMI ITSUNARI (JP)
Application Number:
PCT/JP2021/013099
Publication Date:
October 07, 2021
Filing Date:
March 26, 2021
Export Citation:
Assignee:
CELL EXOSOME THERAPEUTICS INC (JP)
International Classes:
C12N5/071; C07K14/78; C12N5/0775
Domestic Patent References:
| WO2016010082A1 | 2016-01-21 | |||
| WO2014199754A1 | 2014-12-18 | |||
| WO2017082220A1 | 2017-05-18 | |||
| WO2019245050A1 | 2019-12-26 | |||
| WO2012137970A1 | 2012-10-11 | |||
| WO2014103534A1 | 2014-07-03 | |||
| WO2016010082A1 | 2016-01-21 |
Foreign References:
| JPH05207875A | 1993-08-20 | |||
| JPH09512165A | 1997-12-09 | |||
| JP2010538681A | 2010-12-16 | |||
| CN104651305A | 2015-05-27 | |||
| JPH10179147A | 1998-07-07 | |||
| JP2020508679A | 2020-03-26 |
Other References:
STEPHANOPOULOS A. SAMBANIS ET AL.: "Use of Regulated Secretion in Protein Production from Animal Cells: An Evaluation with the AtT-20 Model Cell Line", BIOTECHNOLOGY AND BIOENGINEERING, vol. 35, 1990, pages 771 - 780, XP000673557, DOI: 10.1002/bit.260350804
DE LA GARZA-RODEA ANABEL S., VERWEIJ MARIEKE C., BOERSMA HESTER, VAN DER VELDE-VAN DIJKE IETJE, DE VRIES ANTOINE A. F., HOEBEN ROB: "Exploitation of Herpesvirus Immune Evasion Strategies to Modify the Immunogenicity of Human Mesenchymal Stem Cell Transplants", PLOS ONE, vol. 6, no. 1, 1 January 2011 (2011-01-01), XP055925774, DOI: 10.1371/journal.pone.0014493
ANONYMOUS: "CD9XCD63 ELISA Kit for Human-Derived Exosome Quantitation", COSMO BIO NEWS, no. 140, 1 April 2018 (2018-04-01), JP, pages 15, XP009540547
FUJITA, MIYUKI; YANAGAWA, TOSHIHIDE; IMABAYASHI, TSUKASA; TAKAHASHI, EMI; OYANAGI, JUN; MIYAZAKI, KAORU: "2T10p-18 Effects of various laminin isoforms on growth and differentiation of human mesenchymal stem cells", 84TH ANNUAL MEETING OF THE JAPANESE BIOCHEMICAL SOCIETY; KYOTO, JAPAN; SEPTEMBER 21-24, 2011, vol. 84, 30 November 2010 (2010-11-30) - 24 September 2011 (2011-09-24), pages 2T10p-18, XP009541007
DE LA GARZA-RODEA ANABEL S., VERWEIJ MARIEKE C., BOERSMA HESTER, VAN DER VELDE-VAN DIJKE IETJE, DE VRIES ANTOINE A. F., HOEBEN ROB: "Exploitation of Herpesvirus Immune Evasion Strategies to Modify the Immunogenicity of Human Mesenchymal Stem Cell Transplants", PLOS ONE, vol. 6, no. 1, 1 January 2011 (2011-01-01), XP055925774, DOI: 10.1371/journal.pone.0014493
ANONYMOUS: "CD9XCD63 ELISA Kit for Human-Derived Exosome Quantitation", COSMO BIO NEWS, no. 140, 1 April 2018 (2018-04-01), JP, pages 15, XP009540547
FUJITA, MIYUKI; YANAGAWA, TOSHIHIDE; IMABAYASHI, TSUKASA; TAKAHASHI, EMI; OYANAGI, JUN; MIYAZAKI, KAORU: "2T10p-18 Effects of various laminin isoforms on growth and differentiation of human mesenchymal stem cells", 84TH ANNUAL MEETING OF THE JAPANESE BIOCHEMICAL SOCIETY; KYOTO, JAPAN; SEPTEMBER 21-24, 2011, vol. 84, 30 November 2010 (2010-11-30) - 24 September 2011 (2011-09-24), pages 2T10p-18, XP009541007
Attorney, Agent or Firm:
KURATA, Masatoshi et al. (JP)
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