Title:
METHOD FOR PRODUCING PROLIFERATIVE MACROPHAGE-LIKE CELLS (pMAC)
Document Type and Number:
WIPO Patent Application WO/2024/034656
Kind Code:
A1
Abstract:
The present invention provides: proliferative macrophage-like cells (pMAC), characterized by proliferating in a cytokine-dependent manner; a method for producing the same; and a method for producing chimeric antigen receptor (CAR)-proliferative macrophage-like cells (pMAC) that includes (1) a step (step 1) that deletes or suppresses the expression of a gene that inhibits macrophage-like function in pluripotent stem cells, then induces differentiation into myeloid cells to obtain macrophage-like cells (MAC), (2) a step (step 2) that brings about expression of a gene that adds a proliferative property to the MAC obtained in step 1 and obtains proliferative macrophage-like cells (pMAC), and (3) a step (step 3) that introduces a CAR. The present invention also provides the pharmaceutical use of CAR-pMAC. The present invention makes it possible to provide a novel CAR-loaded cell platform, especially a CAR-loaded cell platform having excellent quality stability, stable supply, and economy, and especially to provide a CAR-loaded cell platform that is excellent as a pharmaceutical.
Inventors:
FUKUDA KYOKO (JP)
UEMURA YASUSHI (JP)
HAGIYA YUICHIRO (JP)
IDE MASAMICHI (JP)
KARIYA MASAHIRO (JP)
UEMURA YASUSHI (JP)
HAGIYA YUICHIRO (JP)
IDE MASAMICHI (JP)
KARIYA MASAHIRO (JP)
Application Number:
PCT/JP2023/029198
Publication Date:
February 15, 2024
Filing Date:
August 09, 2023
Export Citation:
Assignee:
AGC INC (JP)
NAT CANCER CT (JP)
NAT CANCER CT (JP)
International Classes:
C12N5/10; C12N5/02
Domestic Patent References:
| WO2012043651A1 | 2012-04-05 |
Foreign References:
| JP2018503375A | 2018-02-08 | |||
| JP2016028592A | 2016-03-03 | |||
| JP2018521667A | 2018-08-09 | |||
| JP2021511809A | 2021-05-13 |
Other References:
KITANI, H. ; TAKENOUCHI, T. ; SATO, M. ; YOSHIOKA, M. ; YAMANAKA, N.: "A novel isolation method for macrophage-like cells from mixed primary cultures of adult rat liver cells", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM., NL, vol. 360, no. 1-2, 31 August 2010 (2010-08-31), NL , pages 47 - 55, XP027222169, ISSN: 0022-1759
SLOAS CHRISTOPHER, GILL SAAR, KLICHINSKY MICHAEL: "Engineered CAR-Macrophages as Adoptive Immunotherapies for Solid Tumors", FRONTIERS IN IMMUNOLOGY, vol. 12, 24 November 2021 (2021-11-24), XP055964792, DOI: 10.3389/fimmu.2021.783305
IWAMOTO, YOSHIHIRO ET AL.: "iPS Cells and Macrophage", EXPERIMENTAL MEDICINE, YODOSHA CO., LTD., JP, vol. 40, no. 5 Suppl., 1 March 2022 (2022-03-01), JP , pages 19 (655) - 24 (660), XP009552891, ISSN: 0288-5514
SLOAS CHRISTOPHER, GILL SAAR, KLICHINSKY MICHAEL: "Engineered CAR-Macrophages as Adoptive Immunotherapies for Solid Tumors", FRONTIERS IN IMMUNOLOGY, vol. 12, 24 November 2021 (2021-11-24), XP055964792, DOI: 10.3389/fimmu.2021.783305
IWAMOTO, YOSHIHIRO ET AL.: "iPS Cells and Macrophage", EXPERIMENTAL MEDICINE, YODOSHA CO., LTD., JP, vol. 40, no. 5 Suppl., 1 March 2022 (2022-03-01), JP , pages 19 (655) - 24 (660), XP009552891, ISSN: 0288-5514
Attorney, Agent or Firm:
KAMADA, Mitsunori et al. (JP)
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