Title:
METHOD FOR PURIFYING ANTIBODY-LIKE PROTEIN
Document Type and Number:
WIPO Patent Application WO/2017/014261
Kind Code:
A1
Abstract:
Provided is a method for purifying an antibody-like protein, said method including a step for eluting under weakly acidic conditions. This method for purifying an antibody-like protein contains the following steps (a) and (b): (a) a step in which an antibody-like protein is brought into contact with an affinity separation matrix containing a ligand immobilized on a carrier, and is suctioned to the affinity separation matrix; and (b) a step in which an eluate having a pH of at least 3.5 is brought into contact with the affinity separation matrix, and the antibody-like protein is eluted, wherein the ligand contains an amino acid sequence obtained by replacing, in an amino acid sequence derived from the E, D, A, B, or C domain of a protein A described by SEQ ID NO: 1-5, a Gln and/or Lys of an Fc binding site with an Ala, Ser, and/or Thr, and the ligand has a reduced antibody binding capacity in acidic pH regions in comparison with the pre-replacement ligand.
Inventors:
NISHIHACHIJYO MASAKATSU (JP)
NAKANO YOSHIYUKI (JP)
KONOIKE FUMINORI (JP)
TAKANO MASAYUKI (JP)
YOSHIDA SHINICHI (JP)
MINAKUCHI KAZUNOBU (JP)
NAKANO YOSHIYUKI (JP)
KONOIKE FUMINORI (JP)
TAKANO MASAYUKI (JP)
YOSHIDA SHINICHI (JP)
MINAKUCHI KAZUNOBU (JP)
Application Number:
PCT/JP2016/071369
Publication Date:
January 26, 2017
Filing Date:
July 21, 2016
Export Citation:
Assignee:
KANEKA CORP (JP)
International Classes:
C07K1/22; B01J20/281; C07K14/31; C07K16/00; C07K17/02; C12N15/09
Domestic Patent References:
| WO2015005859A1 | 2015-01-15 | |||
| WO2012165544A1 | 2012-12-06 | |||
| WO2013187398A1 | 2013-12-19 | |||
| WO2011118699A1 | 2011-09-29 | |||
| WO2008143199A1 | 2008-11-27 |
Other References:
TSUKAMOTO, M. ET AL.: "Engineered protein A ligands, derived from a histidine-scanning library, facilitate the affinity purification of IgG under mild acidic conditions.", JOURNAL OF BIOLOGICAL ENGINEERING, vol. 8, no. 15, pages 1 - 9, XP021195003, ISSN: 1754-1611
BROWN, N.L. ET AL.: "A study of the interactions between an IgG-binding domain based on the B domain of staphylococcal protein A and rabbit IgG.", MOLECULAR BIOTECHNOLOGY, vol. 10, no. 1, August 1998 (1998-08-01), pages 9 - 16, XP055072952, ISSN: 1073-6085
BROWN, N.L. ET AL.: "A study of the interactions between an IgG-binding domain based on the B domain of staphylococcal protein A and rabbit IgG.", MOLECULAR BIOTECHNOLOGY, vol. 10, no. 1, August 1998 (1998-08-01), pages 9 - 16, XP055072952, ISSN: 1073-6085
Attorney, Agent or Firm:
YASUTOMI & ASSOCIATES (JP)
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