Title:
METHOD FOR QUANTIFYING CHOLESTEROL IN HIGH-DENSITY LIPOPROTEIN
Document Type and Number:
WIPO Patent Application WO/2019/177093
Kind Code:
A1
Abstract:
Disclosed are: a method for quantifying cholesterol in a HDL, whereby it becomes possible to selectively, simply and correctly quantify HDL cholesterol in a sample of interest containing an HDL as well as another lipoprotein such as a LDL without requiring any complicated fractionation/separation procedure; and a method for selectively eliminating cholesterol, which can be employed in the aforementioned method. A method for eliminating cholesterol in a lipoprotein other than a high-density lipoprotein in a sample of interest out of a reaction system includes allowing (i) phospholipase and/or sphingomyelinase and (ii) a nonionic surfactant to act on the sample.
Inventors:
UCHIDA SHIGENORI (JP)
HIRAO YUHKO (JP)
HIRAO YUHKO (JP)
Application Number:
PCT/JP2019/010525
Publication Date:
September 19, 2019
Filing Date:
March 14, 2019
Export Citation:
Assignee:
DENKA SEIKEN KK (JP)
International Classes:
C12Q1/60; C12M1/34; C12Q1/26; C12Q1/34; C12Q1/42; C12Q1/44; G01N33/92
Domestic Patent References:
| WO2012011554A1 | 2012-01-26 | |||
| WO1998026090A1 | 1998-06-18 |
Foreign References:
| JPH09299A | 1997-01-07 |
Other References:
NORIAKI ET AL.: "Development of a direct method of new HDL subfraction cholesterol", JAPANESE JOURNAL OF OF CLINICAL LABORATORY AUTOMATION, vol. 40, no. 3, 2015, SATOH, pages 198 - 204, ISSN: 0286-1607
Attorney, Agent or Firm:
TANIGAWA AND PARTNERS, PATENT FIRM (JP)
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