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Title:
METHOD FOR QUANTIFYING VITAMIN D DERIVATIVES, ENZYME FOR QUANTIFICATION, COMPOSITION FOR QUANTIFICATION, KIT FOR QUANTIFICATION, ELECTRODE, SENSOR CHIP, AND SENSOR
Document Type and Number:
WIPO Patent Application WO/2021/117790
Kind Code:
A1
Abstract:
Provided are a new method for quantifying vitamin D derivatives, an enzyme for quantification, a composition for quantification, a kit for quantification, an electrode, a sensor chip, and a sensor. Provided is a method for quantifying vitamin D derivatives, the method including adding an oxidoreductase into a sample. The concentration of vitamin D derivatives may be determined by reducing a mediator through the addition of the oxidoreductase and then reacting the reduced mediator with a reagent. The oxidoreductase is an oxidase, and the concentration of vitamin D derivatives may be determined by quantifying the hydrogen peroxide produced through the addition of the oxidase or quantifying the consumed oxygen. In addition, the concentration of vitamin D derivatives may be determined by reacting a reagent and the hydrogen peroxide produced through the addition of the oxidase.

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Inventors:
HAYASHI KANAKO (JP)
ICHIYANAGI ATSUSHI (JP)
Application Number:
PCT/JP2020/045938
Publication Date:
June 17, 2021
Filing Date:
December 09, 2020
Export Citation:
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Assignee:
KIKKOMAN CORP (JP)
International Classes:
C12N9/04; G01N33/52; G01N33/82
Domestic Patent References:
WO2002073181A12002-09-19
Foreign References:
JP2016531306A2016-10-06
Other References:
ABDEL-KHALIK, J. ET AL.: "Studies on the analysis of 25-hydroxyvitamin D3 by isotope-dilution liquid chromatography-tandem mass spectrometry using enzyme-assisted derivatisation", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 446, 30 January 2014 (2014-01-30), pages 745 - 750, XP055835112
ARBOUR, N. C. ET AL.: "A Highly Sensitive Method for Large-Scale Measurements of 1, 25- Dihydroxyvitamin D", ANALYTICAL BIOCHEMISTRY, vol. 255, no. 1, 1 January 1998 (1998-01-01), pages 148 - 154, XP055835113
BURGOS-TRINIDAD, M. ET AL.: "A Rapid Assay for 25- Hydroxyvitamin D and 1, 25-Dihydroxyvitamin D 24- Hydroxylase", ANALYTICAL BIOCHEMISTRY, vol. 190, no. 1, October 1990 (1990-10-01), pages 102 - 107, XP024817105, DOI: 10.1016/0003-2697(90)90141-U
Attorney, Agent or Firm:
TAKAHASHI, HAYASHI AND PARTNER PATENT ATTORNEYS, INC. (JP)
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