Title:
METHOD FOR SCREENING FOR CANDIDATE MOLECULE CAPABLE OF FORMING COMPLEX IN CONJUNCTION WITH PLURALITY OF TARGET MOLECULES
Document Type and Number:
WIPO Patent Application WO/2022/138892
Kind Code:
A1
Abstract:
A method for screening for a molecule capable of forming a complex in conjunction with a plurality of target molecules by applying a candidate molecule collection method utilizing an affinity technique in combination with a technique typified by a Split GFP technique is discovered. In this method, a first target molecule linked to a first segment in a protein, a second target molecule linked to a second segment in the protein, and a library including a plurality of test molecules are allowed to coexist. A test molecule which can form a complex in conjunction with the first target molecule linked to the first segment and the second target molecule linked to the second segment is collected by utilizing an affinity technique.
Inventors:
KOJIMA TETSUO (JP)
NISHIMURA KAORI (JP)
KURIKI YUGO (JP)
NISHIMURA KAORI (JP)
KURIKI YUGO (JP)
Application Number:
PCT/JP2021/048097
Publication Date:
June 30, 2022
Filing Date:
December 24, 2021
Export Citation:
Assignee:
CHUGAI PHARMACEUTICAL CO LTD (JP)
International Classes:
G01N33/537; C12N15/12; C12N15/53; C12Q1/6869; G01N33/15; G01N33/50; G01N33/68
Domestic Patent References:
| WO2013100132A1 | 2013-07-04 | |||
| WO2018225864A1 | 2018-12-13 | |||
| WO2017150732A1 | 2017-09-08 | |||
| WO2016148044A1 | 2016-09-22 | |||
| WO2020138336A1 | 2020-07-02 | |||
| WO2018143145A1 | 2018-08-09 | |||
| WO2020138336A1 | 2020-07-02 |
Foreign References:
| US20150099271A1 | 2015-04-09 | |||
| JP2008521447A | 2008-06-26 | |||
| CN110283244A | 2019-09-27 | |||
| US20040053388A1 | 2004-03-18 | |||
| US9428845B1 | 2016-08-30 | |||
| US20180164324A1 | 2018-06-14 | |||
| US20100281552A1 | 2010-11-04 | |||
| US20120174242A1 | 2012-07-05 | |||
| US20180172692A1 | 2018-06-21 |
Other References:
MIE MASAYASU, NAOKI TATSUHIKO, KOBATAKE EIRY: "Tracking a protein following dissociation from a protein–protein complex using a split SNAP-tag system", ANALYTICAL BIOCHEMISTRY, vol. 477, 15 May 2015 (2015-05-15), Amsterdam, NL , pages 53 - 55, XP055945699, ISSN: 0003-2697, DOI: 10.1016/j.ab.2015.02.019
HÖRING CARINA, SEIBEL ULLA, TROPMANN KATHARINA, GRÄTZ LUKAS, MÖNNICH DENISE, PITZL SEBASTIAN, BERNHARDT GÜNTHER, POCKES STEFFEN, S: "A Dynamic, Split-Luciferase-Based Mini-G Protein Sensor to Functionally Characterize Ligands at All Four Histamine Receptor Subtypes", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 21, no. 22, 1 January 2020 (2020-01-01), pages 1 - 18, XP055945700, DOI: 10.3390/ijms21228440
ULRICH, H. W. ET AL., CANCER GENOMICS & PROTEOMICS, vol. 10, 2013, pages 1 - 18
CABANTOUS, S. ET AL., SCI REP., vol. 3, 2013, pages 2854
KORAICHI, F. ET AL., JOURNAL OF CELL SCIENCE, 2018, pages 131
PROTEIN ENGINEERING, vol. 9, no. 3, 1996, pages 299 - 305
PROC NATL ACAD SCI USA., vol. 94, 1997, pages 12297 - 302
FEBS LETT., vol. 414, 1997, pages 405 - 8
NUCLEIC ACIDS RES., vol. 37, no. 16, 2009, pages e108
PROC NATL ACAD SCI USA., vol. 91, 1994, pages 9022 - 6
NUCLEIC ACIDSRES., vol. 33, 2005, pages el0
PROC NATL ACAD SCI USA., vol. 101, 2004, pages 2806 - 10
NAT BIOTECHNOL., vol. 16, 1998, pages 652 - 6
WEN-XUE J. ET AL., SCI REP., vol. 6, 2016, pages 20568
ANALYST, vol. 137, 2012, pages 4760 - 4765
ANAL. CHEM., vol. 88, 2016, pages 8166 - 8171
PNAS, vol. 104, no. 27, 3 July 2007 (2007-07-03), pages 11209 - 11214
ANDREW, D. ET AL., SCI REP., vol. 7, 2017, pages 8186
ANAL. CHEM., vol. 90, 2018, pages 3001 - 3004
KUNKEL ET AL., PROC. NATL. ACAD. SCI. USA, vol. 82, 1985, pages 488 - 492
CARLINARTHUR, PROC. NATL. ACAD. SCI. USA, vol. 87, 1990, pages 2264 - 2268
PROC NATL ACAD SCI USA, vol. 90, 1993, pages 5873
ALTSCHUL SF ET AL., J MOL BIOL, vol. 215, 1990, pages 403
W. AND FRITZ, H. -J.: "Oligonucleotide-directed construction of mutagenesis via gapped duplex DNA", METHODS IN ENZYMOLOGY, vol. 154, 1987, pages 350 - 367
SOUTHERN, E.M., JOURNAL OF MOLECULAR BIOLOGY, vol. 98, 1975, pages 503
MANDEL, M.HIGA, A., JOURNAL OF MOLECULAR BIOLOGY, vol. 53, 1970, pages 158 - 162
HANAHAN, D., JOURNAL OF MOLECULAR BIOLOGY, vol. 166, 1983, pages 557 - 580
WARD ET AL., NATURE, vol. 341, 1989, pages 544 - 546
FASEB J., vol. 6, 1992, pages 2422 - 2427
BETTER ET AL., SCIENCE, vol. 240, 1988, pages 1041 - 1043
LEI, S. P. ET AL., J. BACTERIOL., vol. 169, 1987, pages 4379
MULLIGAN ET AL., NATURE, vol. 277, 1979, pages 108
MIZUSHIMA ET AL., NUCLEIC ACIDS RES., vol. 18, 1990, pages 5322
VALLE ET AL., NATURE, vol. 291, 1981, pages 358 - 340
PROC. NATL. ACAD. SCI. USA, vol. 77, 1980, pages 4216 - 4220
PROC. NATL. ACAD. SCI. USA, vol. 60, 1968, pages 1275
J AM CHEM SOC., vol. 127, 2005, pages 4715 - 21
PROC. NATL. ACAD. SCI. USA., vol. 108, no. 28, 12 July 2011 (2011-07-12), pages 11399 - 11404
HÖRING CARINA, SEIBEL ULLA, TROPMANN KATHARINA, GRÄTZ LUKAS, MÖNNICH DENISE, PITZL SEBASTIAN, BERNHARDT GÜNTHER, POCKES STEFFEN, S: "A Dynamic, Split-Luciferase-Based Mini-G Protein Sensor to Functionally Characterize Ligands at All Four Histamine Receptor Subtypes", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 21, no. 22, 1 January 2020 (2020-01-01), pages 1 - 18, XP055945700, DOI: 10.3390/ijms21228440
ULRICH, H. W. ET AL., CANCER GENOMICS & PROTEOMICS, vol. 10, 2013, pages 1 - 18
CABANTOUS, S. ET AL., SCI REP., vol. 3, 2013, pages 2854
KORAICHI, F. ET AL., JOURNAL OF CELL SCIENCE, 2018, pages 131
PROTEIN ENGINEERING, vol. 9, no. 3, 1996, pages 299 - 305
PROC NATL ACAD SCI USA., vol. 94, 1997, pages 12297 - 302
FEBS LETT., vol. 414, 1997, pages 405 - 8
NUCLEIC ACIDS RES., vol. 37, no. 16, 2009, pages e108
PROC NATL ACAD SCI USA., vol. 91, 1994, pages 9022 - 6
NUCLEIC ACIDSRES., vol. 33, 2005, pages el0
PROC NATL ACAD SCI USA., vol. 101, 2004, pages 2806 - 10
NAT BIOTECHNOL., vol. 16, 1998, pages 652 - 6
WEN-XUE J. ET AL., SCI REP., vol. 6, 2016, pages 20568
ANALYST, vol. 137, 2012, pages 4760 - 4765
ANAL. CHEM., vol. 88, 2016, pages 8166 - 8171
PNAS, vol. 104, no. 27, 3 July 2007 (2007-07-03), pages 11209 - 11214
ANDREW, D. ET AL., SCI REP., vol. 7, 2017, pages 8186
ANAL. CHEM., vol. 90, 2018, pages 3001 - 3004
KUNKEL ET AL., PROC. NATL. ACAD. SCI. USA, vol. 82, 1985, pages 488 - 492
CARLINARTHUR, PROC. NATL. ACAD. SCI. USA, vol. 87, 1990, pages 2264 - 2268
PROC NATL ACAD SCI USA, vol. 90, 1993, pages 5873
ALTSCHUL SF ET AL., J MOL BIOL, vol. 215, 1990, pages 403
W. AND FRITZ, H. -J.: "Oligonucleotide-directed construction of mutagenesis via gapped duplex DNA", METHODS IN ENZYMOLOGY, vol. 154, 1987, pages 350 - 367
SOUTHERN, E.M., JOURNAL OF MOLECULAR BIOLOGY, vol. 98, 1975, pages 503
MANDEL, M.HIGA, A., JOURNAL OF MOLECULAR BIOLOGY, vol. 53, 1970, pages 158 - 162
HANAHAN, D., JOURNAL OF MOLECULAR BIOLOGY, vol. 166, 1983, pages 557 - 580
WARD ET AL., NATURE, vol. 341, 1989, pages 544 - 546
FASEB J., vol. 6, 1992, pages 2422 - 2427
BETTER ET AL., SCIENCE, vol. 240, 1988, pages 1041 - 1043
LEI, S. P. ET AL., J. BACTERIOL., vol. 169, 1987, pages 4379
MULLIGAN ET AL., NATURE, vol. 277, 1979, pages 108
MIZUSHIMA ET AL., NUCLEIC ACIDS RES., vol. 18, 1990, pages 5322
VALLE ET AL., NATURE, vol. 291, 1981, pages 358 - 340
PROC. NATL. ACAD. SCI. USA, vol. 77, 1980, pages 4216 - 4220
PROC. NATL. ACAD. SCI. USA, vol. 60, 1968, pages 1275
J AM CHEM SOC., vol. 127, 2005, pages 4715 - 21
PROC. NATL. ACAD. SCI. USA., vol. 108, no. 28, 12 July 2011 (2011-07-12), pages 11399 - 11404
Attorney, Agent or Firm:
HASEGAWA, Yoshiki et al. (JP)
Download PDF: