Title:
METHOD FOR SCREENING VASCULAR ENDOTHELIAL CELL ACTIVATION SUBSTANCES
Document Type and Number:
WIPO Patent Application WO/2021/029436
Kind Code:
A1
Abstract:
The present invention relates to a method which screens substances for promoting or inhibiting vascular endothelial activation and uses, as an index, the uptake quantity of VEGF into vascular endothelial cells. More specifically, the present invention relates to a method for screening substances for promoting or inhibiting vascular endothelial activation, the method comprising the following steps of: 1) culturing vascular endothelial cells along with test substances; 2) determining the uptake quantity of VEGF into the vascular endothelial cells; and 3) evaluating the vascular endothelial cell activation effect of the test substances on the basis of the uptake quantity of VEGF into the vascular endothelial cells.
Inventors:
TAGUCHI AKIHIKO (JP)
TAURA AKIE (JP)
OGAWA YUKO (JP)
TAURA AKIE (JP)
OGAWA YUKO (JP)
Application Number:
PCT/JP2020/030921
Publication Date:
February 18, 2021
Filing Date:
August 04, 2020
Export Citation:
Assignee:
FOUNDATION FOR BIOMEDICAL RES AND INNOVATION AT KOBE (JP)
International Classes:
C12Q1/02; G01N33/15; G01N33/50; G01N33/68
Foreign References:
| JP2012502114A | 2012-01-26 |
Other References:
NAKANO-DOI AKIKO, NAKAGOMI TAKAYUKI, FUJIKAWA MASATOSHI, NAKAGOMI NAMI, KUBO SHUJI, LU SHAN, YOSHIKAWA HIROO, SOMA TOSHIHIRO, TAGU: "Bone marrow mononuclear cells promote proliferation of endogenous neural stem cells through vascular niches after cerebral infarction", STEM CELLS, vol. 28, no. 7, 2010, pages 1292 - 1302, XP055793467
TAGUCHI, AKIHIKO: "Therapeutic angiogenesis for cerebrovascular diseases with hematopoietic stem cells", NEUROLOGICAL THERAPEUTICS, vol. 34, no. 1, 2017, pages 31 - 36, XP055793471
WANG JIANPING; FU XIAOJIE; YU LIE; LI NAN; WANG MENGHAN; LIU XI; ZHANG DI; HAN WEI; ZHOU CHENGUANG; WANG JIAN: "Preconditioning with VEGF Enhances Angiogenic and Neuroprotective Effects of Bone Marrow Mononuclear Cell Transplantation in a Rat Model of Chronic Cerebral Hypoperfusion", MOLECULAR NEUROBIOLOGY, vol. 53, no. 9, 2016, pages 6057 - 6068, XP036087266
FUKUHARA, SHIGETOMO: "Signaling mechanisms underlying dynamic regulation of vascular permeability", JOURNAL OF JAPANESE BIOCHEMICAL SOCIETY, vol. 89, no. 3, 2017, pages 368 - 376
WEISE, S. M. ET AL.: "Pathophysiological consequences of VEGF-induced vascular permeability", NATURE, vol. 437, no. 7058, 22 September 2005 (2005-09-22), pages 497 - 504, XP002688930, DOI: 10.1038/NATURE03987
KIKUCHI-TAURA AKIE, OKINAKA YUKA, TAKEUCHI YUKIKO, OGAWA YUKO, MAEDA MITSUYO, KATAOKA YOSKY, YASUI TERUHITO, KIMURA TAKAFUMI, GUL : "Bone Marrow Mononuclear Cells Activate Angiogenesis via Gap Junction- Mediated Cell - Cell Interaction", STROKE, vol. 51, no. 4, 19 February 2020 (2020-02-19), pages 1279 - 1289, XP055793488
TAGUCHI, AKIHIKO: "Therapeutic angiogenesis for cerebrovascular diseases with hematopoietic stem cells", NEUROLOGICAL THERAPEUTICS, vol. 34, no. 1, 2017, pages 31 - 36, XP055793471
WANG JIANPING; FU XIAOJIE; YU LIE; LI NAN; WANG MENGHAN; LIU XI; ZHANG DI; HAN WEI; ZHOU CHENGUANG; WANG JIAN: "Preconditioning with VEGF Enhances Angiogenic and Neuroprotective Effects of Bone Marrow Mononuclear Cell Transplantation in a Rat Model of Chronic Cerebral Hypoperfusion", MOLECULAR NEUROBIOLOGY, vol. 53, no. 9, 2016, pages 6057 - 6068, XP036087266
FUKUHARA, SHIGETOMO: "Signaling mechanisms underlying dynamic regulation of vascular permeability", JOURNAL OF JAPANESE BIOCHEMICAL SOCIETY, vol. 89, no. 3, 2017, pages 368 - 376
WEISE, S. M. ET AL.: "Pathophysiological consequences of VEGF-induced vascular permeability", NATURE, vol. 437, no. 7058, 22 September 2005 (2005-09-22), pages 497 - 504, XP002688930, DOI: 10.1038/NATURE03987
KIKUCHI-TAURA AKIE, OKINAKA YUKA, TAKEUCHI YUKIKO, OGAWA YUKO, MAEDA MITSUYO, KATAOKA YOSKY, YASUI TERUHITO, KIMURA TAKAFUMI, GUL : "Bone Marrow Mononuclear Cells Activate Angiogenesis via Gap Junction- Mediated Cell - Cell Interaction", STROKE, vol. 51, no. 4, 19 February 2020 (2020-02-19), pages 1279 - 1289, XP055793488
Attorney, Agent or Firm:
OHNO Seiji et al. (JP)
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