Title:
METHOD FOR SIMULTANEOUSLY ENHANCING AND INHIBITING MULTIPLE KEY GENES DURING SYNTHESIS OF 7-DEHYDROCHOLESTEROL IN SACCHAROMYCES CEREVISIAE
Document Type and Number:
WIPO Patent Application WO/2023/173565
Kind Code:
A1
Abstract:
Provided is a method for simultaneously enhancing and inhibiting multiple key genes during synthesis of 7-dehydrocholesterol in Saccharomyces cerevisiae by means of a dCpf1-VP activation system and a dCas9-RD inhibition system. In order to achieve the aim of simultaneous expression and inhibition of 13 P450 enzymes, an engineered yeast for producing 7-dehydrocholesterol at a high yield is constructed, so that the 7-DHC yield reaches 464 mg/L.
Inventors:
LIU LONG (CN)
CHEN JIAN (CN)
LV XUEQIN (CN)
DU GUOCHENG (CN)
LI JIANGHUA (CN)
LIU YANFENG (CN)
XIU XIANG (CN)
CHEN JIAN (CN)
LV XUEQIN (CN)
DU GUOCHENG (CN)
LI JIANGHUA (CN)
LIU YANFENG (CN)
XIU XIANG (CN)
Application Number:
PCT/CN2022/092329
Publication Date:
September 21, 2023
Filing Date:
May 12, 2022
Export Citation:
Assignee:
UNIV JIANGNAN (CN)
International Classes:
C12N1/19; C12N9/02; C12N9/04; C12N9/10; C12N9/90; C12N15/53; C12N15/54; C12N15/61; C12N15/81; C12P33/02; C12R1/865
Foreign References:
| CN112877230A | 2021-06-01 | |||
| CN104988168A | 2015-10-21 | |||
| CN113025512A | 2021-06-25 | |||
| CN113151027A | 2021-07-23 | |||
| CN112813129A | 2021-05-18 |
Other References:
WEN-QIAN ZHANG, XIAO WEN-HAI, ZHOU XIAO, WANG YING: "Effect of Post-squalene Genes on the Synthesis of 7-Dehydrocholesterol in the Artificial Saccharomyces cerevisiae", CHINA BIOTECHNOLOGY, vol. 36, no. 6, 15 June 2016 (2016-06-15), pages 39 - 50, XP093091748
XIAO-JING GUO, WEN-HAI XIAO, YING WANG, MING-DONG YAO, BO-XUAN ZENG, HONG LIU, GUANG-RONG ZHAO, YING-JIN YUAN: "Metabolic engineering of Saccharomyces cerevisiae for 7-dehydrocholesterol overproduction", BIOTECHNOLOGY FOR BIOFUELS, vol. 11, no. 1, 1 December 2018 (2018-12-01), XP055615428, DOI: 10.1186/s13068-018-1194-9
CHEN YUN, SIEWERS VERENA, NIELSEN JENS: "Profiling of Cytosolic and Peroxisomal Acetyl-CoA Metabolism in Saccharomyces cerevisiae", PLOS ONE, vol. 7, no. 8, 2 August 2012 (2012-08-02), pages e42475, XP093091712, DOI: 10.1371/journal.pone.0042475
XIAO-JING GUO, WEN-HAI XIAO, YING WANG, MING-DONG YAO, BO-XUAN ZENG, HONG LIU, GUANG-RONG ZHAO, YING-JIN YUAN: "Metabolic engineering of Saccharomyces cerevisiae for 7-dehydrocholesterol overproduction", BIOTECHNOLOGY FOR BIOFUELS, vol. 11, no. 1, 1 December 2018 (2018-12-01), XP055615428, DOI: 10.1186/s13068-018-1194-9
CHEN YUN, SIEWERS VERENA, NIELSEN JENS: "Profiling of Cytosolic and Peroxisomal Acetyl-CoA Metabolism in Saccharomyces cerevisiae", PLOS ONE, vol. 7, no. 8, 2 August 2012 (2012-08-02), pages e42475, XP093091712, DOI: 10.1371/journal.pone.0042475
Attorney, Agent or Firm:
CENTRAL SOUTH WELL INTELLECTUAL PROPERTY OFFICE (CN)
Download PDF: