Title:
SACCHAROMYCES CEREVISIAE FOR PRODUCING RUBUSOSIDE AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/173566
Kind Code:
A1
Abstract:
The present invention relates to saccharomyces cerevisiae for producing rubusoside and an application thereof. A rubusoside synthesis gene is expressed in a saccharomyces cerevisiae strain, and an ABC efflux pump is overexpressed; and the ABC efflux pump is selected from at least one of YOR1, PDR11, and PDR12. According to the present invention, a rubusoside synthesis path is constructed in the saccharomyces cerevisiae, so that the saccharomyces cerevisiae can generate rubusoside, and recombinant saccharomyces cerevisiae is modified by further applying promoter engineering and genetic engineering, so that de novo synthesized rubusoside having glucose as a substrate is achieved, and the recombinant saccharomyces cerevisiae has good performance in fermentation production of rubusoside.
Inventors:
LIU LONG (CN)
CHEN JIAN (CN)
LV XUEQIN (CN)
DU GUOCHENG (CN)
LI JIANGHUA (CN)
LIU YANFENG (CN)
XU YAMENG (CN)
CHEN JIAN (CN)
LV XUEQIN (CN)
DU GUOCHENG (CN)
LI JIANGHUA (CN)
LIU YANFENG (CN)
XU YAMENG (CN)
Application Number:
PCT/CN2022/092330
Publication Date:
September 21, 2023
Filing Date:
May 12, 2022
Export Citation:
Assignee:
UNIV JIANGNAN (CN)
International Classes:
C12N1/19; C12N15/31; C12N15/53; C12N15/54; C12N15/60; C12N15/61; C12N15/81; C12P19/56; C12R1/865
Domestic Patent References:
| WO2018211032A1 | 2018-11-22 |
Foreign References:
| US20150361476A1 | 2015-12-17 | |||
| US20200347425A1 | 2020-11-05 | |||
| CN112424218A | 2021-02-26 | |||
| CN107109358A | 2017-08-29 | |||
| US20210238235A1 | 2021-08-05 | |||
| CN112852653A | 2021-05-28 | |||
| CN112867799A | 2021-05-28 |
Other References:
ZHOU ZHOUYU, SUI XIAOCHEN, XIA YONGMEI: "Enzymatic Synthesis of Rubusoside and Its Inhibition on Liver and Gastrointestinal Cells", CHINESE JOURNAL OF APPLIED CHEMISTRY, vol. 37, no. 7, 1 July 2020 (2020-07-01), pages 785 - 792, XP093089987
XU YAMENG, WANG XINGLONG, ZHANG CHENYANG, ZHOU XUAN, XU XIANHAO, HAN LUYAO, LV XUEQIN, LIU YANFENG, LIU SONG, LI JIANGHUA, DU GUOC: "De novo biosynthesis of rubusoside and rebaudiosides in engineered yeasts", NATURE COMMUNICATIONS, vol. 13, no. 1, XP093089988, DOI: 10.1038/s41467-022-30826-2
XU YAMENG, WANG XINGLONG, ZHANG CHENYANG, ZHOU XUAN, XU XIANHAO, HAN LUYAO, LV XUEQIN, LIU YANFENG, LIU SONG, LI JIANGHUA, DU GUOC: "De novo biosynthesis of rubusoside and rebaudiosides in engineered yeasts", NATURE COMMUNICATIONS, vol. 13, no. 1, XP093089988, DOI: 10.1038/s41467-022-30826-2
Attorney, Agent or Firm:
CENTRAL SOUTH WELL INTELLECTUAL PROPERTY OFFICE (CN)
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