Title:
METHODS AND COMPOSITIONS FOR ENHANCING RISC ACTIVITY IN VITRO AND IN VIVO
Document Type and Number:
WIPO Patent Application WO2005079532
Kind Code:
A3
Abstract:
The present invention provides methods of enhancing the efficacy and specificity of RNAi by priming RISC activity in cells, cell extracts, and organisms using priming agents such as siRNAs as well as other nucleic acids. The invention also provides priming agents, extracts and cells with high levels of primed RISC activity and therefore responsiveness to RNAi, and methods of using the same in research, diagnostic, and therapeutic applications.
Inventors:
RANA TARIQ M (US)
Application Number:
PCT/US2005/005500
Publication Date:
November 09, 2006
Filing Date:
February 17, 2005
Export Citation:
Assignee:
UNIV MASSACHUSETTS (US)
RANA TARIQ M (US)
RANA TARIQ M (US)
International Classes:
C12Q1/68; A01K67/00; A01N43/04; A61K31/07; C07H21/04; C12N5/08; C12N15/11; C12N15/85
Other References:
CHIU Y.-L. ET AL.: "siRNA function in RNAi: A chemical modification analysis", RNA, vol. 9, no. 9, 2003, pages 1034 - 1048, XP002996108
MARTINEZ J. ET AL.: "Single-stranded antisense siRNAs Guide target RNA cleavage in RNAi", CELL, vol. 110, no. 5, 2002, pages 563 - 574, XP002257819
ARENDT C.W. ET AL.: "Vector systems for the delivery of small interfering RNAs: Managing the RISC", CHEMBIOCHEM, vol. 4, 2003, pages 1129 - 1136, XP002976674
CAPLEN N.: "RNAi as a gene therapy approach", GENE THERAPY, vol. 3, no. 4, 2003, pages 575 - 586, XP008055014
COBURN G.A. ET AL.: "siRNAs: a new wave of RNA-based therapeutics", CHEMOTHERAPY, vol. 51, no. 4, 2003, pages 753 - 756, XP002992788
AGAMI R.: "RNAi and related mechanisms and their potential use for therapy", CURR. OPIN. IN CHEM. BIO., vol. 6, 2002, pages 829 - 834, XP002239988
MARTINEZ J. ET AL.: "Single-stranded antisense siRNAs Guide target RNA cleavage in RNAi", CELL, vol. 110, no. 5, 2002, pages 563 - 574, XP002257819
ARENDT C.W. ET AL.: "Vector systems for the delivery of small interfering RNAs: Managing the RISC", CHEMBIOCHEM, vol. 4, 2003, pages 1129 - 1136, XP002976674
CAPLEN N.: "RNAi as a gene therapy approach", GENE THERAPY, vol. 3, no. 4, 2003, pages 575 - 586, XP008055014
COBURN G.A. ET AL.: "siRNAs: a new wave of RNA-based therapeutics", CHEMOTHERAPY, vol. 51, no. 4, 2003, pages 753 - 756, XP002992788
AGAMI R.: "RNAi and related mechanisms and their potential use for therapy", CURR. OPIN. IN CHEM. BIO., vol. 6, 2002, pages 829 - 834, XP002239988
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