Title:
METHODS AND COMPOSITIONS FOR MEDIATING GENE SILENCING
Document Type and Number:
WIPO Patent Application WO2005079533
Kind Code:
A3
Abstract:
The present invention provides methods of conducting RNAi using siRNAs that are sequentially administered as single-stranded oligonucleotides. The siRNAs can be canonical or have non-canonical ends. The compositions and methods of the invention can bypass activation of interferon pathways and yet still efficiently and specifically activate RNAi / gene silencing. In another embodiment, the siRNAs of the invention are modified to allow for the calculation of certain RNAi activities, e.g., RISC activity. The invention also provides methods of using the compositions in research, diagnostic, and therapeutic applications.
Inventors:
RANA TARIQ M (US)
Application Number:
PCT/US2005/005503
Publication Date:
May 26, 2006
Filing Date:
February 17, 2005
Export Citation:
Assignee:
UNIV MASSACHUSETTS (US)
RANA TARIQ M (US)
RANA TARIQ M (US)
International Classes:
C12N15/11; A61K48/00; C07H21/04; C12Q1/68
Foreign References:
| US20040005593A1 | 2004-01-08 |
Other References:
ELBASHIR ET AL: "Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate.", THE EMBO JOURNAL., vol. 20, no. 23, 2001, pages 6877 - 6888, XP002225998
FIRE.: "RNA-triggered gene silencing.", TIG., vol. 15, no. 9, September 1999 (1999-09-01), pages 358 - 363, XP004176656
FIRE.: "RNA-triggered gene silencing.", TIG., vol. 15, no. 9, September 1999 (1999-09-01), pages 358 - 363, XP004176656
Download PDF:
Previous Patent: METHODS AND COMPOSITIONS FOR ENHANCING RISC ACTIVITY IN VITRO AND IN VIVO
Next Patent: SYSTEMS AND METHODS FOR PARALLEL COMMUNICATION
Next Patent: SYSTEMS AND METHODS FOR PARALLEL COMMUNICATION