Title:
METHODS OF IN-SOLUTION POSITIONAL CO-BARCODING FOR SEQUENCING LONG DNA MOLECULES
Document Type and Number:
WIPO Patent Application WO/2024/022207
Kind Code:
A1
Abstract:
The methods and compositions disclosed herein relate to preparing libraries to sequence long molecules in their entirety using massively parallel short read sequencing. The methods disclosed herein generate a nested set of nucleic acid constructs for each genomic fragment and generate a plurality of nested sets for a plurality of genomic fragments. The nucleic acid constructs may be single-stranded or double-stranded. Each nucleic acid construct in each nested set comprises a barcode and target sequence portion, and nucleic acid constructs within each nested set have different lengths. The nucleic acid constructs in each nested set share a unique barcode sequence.
Inventors:
ALEXEEV ANDREI (US)
PETERS BROCK (US)
DRMANAC RADOJE (US)
PETERS BROCK (US)
DRMANAC RADOJE (US)
Application Number:
PCT/CN2023/108314
Publication Date:
February 01, 2024
Filing Date:
July 20, 2023
Export Citation:
Assignee:
MGI TECH CO LTD (CN)
ALEXEEV ANDREI (US)
ALEXEEV ANDREI (US)
International Classes:
C12Q1/68; C12N15/10
Domestic Patent References:
| WO2009017678A2 | 2009-02-05 | |||
| WO2019033062A2 | 2019-02-14 |
Foreign References:
| CN112639094A | 2021-04-09 | |||
| CN109072294A | 2018-12-21 | |||
| US20120003657A1 | 2012-01-05 |
Other References:
OU WANG, ROBERT CHIN, XIAOFANG CHENG, MICHELLE KA YAN WU, QING MAO, JINGBO TANG, YUHUI SUN, ELLIS ANDERSON, HAN K. LAM, DAN CHEN, : "Efficient and unique cobarcoding of second-generation sequencing reads from long DNA molecules enabling cost-effective and accurate sequencing, haplotyping, and de novo assembly", GENOME RESEARCH, COLD SPRING HARBOR LABORATORY PRESS, US, vol. 29, no. 5, 1 May 2019 (2019-05-01), US , pages 798 - 808, XP055630335, ISSN: 1088-9051, DOI: 10.1101/gr.245126.118
WANG LIN, XI YANG, ZHANG WENWEI, WANG WEIMAO, SHEN HANJIE, WANG XIAOJUE, ZHAO XIA, ALEXEEV ANDREI, PETERS BROCK A, ALBERT ALAYNA, : "3′ Branch ligation: a novel method to ligate non-complementary DNA to recessed or internal 3′OH ends in DNA or RNA", DNA RESEARCH, UNIVERSAL ACADEMY PRESS, JP, vol. 26, no. 1, 1 February 2019 (2019-02-01), JP , pages 45 - 53, XP055862729, ISSN: 1340-2838, DOI: 10.1093/dnares/dsy037
WANG LIN, XI YANG, ZHANG WENWEI, WANG WEIMAO, SHEN HANJIE, WANG XIAOJUE, ZHAO XIA, ALEXEEV ANDREI, PETERS BROCK A, ALBERT ALAYNA, : "3′ Branch ligation: a novel method to ligate non-complementary DNA to recessed or internal 3′OH ends in DNA or RNA", DNA RESEARCH, UNIVERSAL ACADEMY PRESS, JP, vol. 26, no. 1, 1 February 2019 (2019-02-01), JP , pages 45 - 53, XP055862729, ISSN: 1340-2838, DOI: 10.1093/dnares/dsy037
Attorney, Agent or Firm:
SYNCLAW ATTORNEYS (CN)
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