Title:
METHODS FOR PRODUCING BIOLOGICAL SUBSTANCES IN ENZYME-DEFICIENT MUTANTS OF ASPERGILLUS NIGER
Document Type and Number:
WIPO Patent Application WO2004090155
Kind Code:
A3
Abstract:
The present invention relates to methods of producing a heterologous biological substance, comprising: (a) cultivating a mutant of a parent Aspergillus niger strain in a medium suitable for the production of the heterologous biological substance, wherein (i) the mutant strain comprises a first nucleotide sequence encoding the heterologous biological substance and one or more second nucleotide sequences comprising a modification of glaA and at least one of the genes selected from the group consisting of asa, amyA, amyB, prtT, and oah, and (ii) the mutant strain is deficient in the production of glucoamylase and at least one enzyme selected from the group consisting of acid stable alpha-amylase, neutral alpha-amylase A, and neutral alpha-amylase B, protease, and oxalic acid hydrolase compared to the parent Aspergillus niger strain when cultivated under identical conditions; and (b) recovering the heterologous biological substance from the cultivation medium.
Inventors:
CONNELLY MARIAH (US)
BRODY HOWARD (US)
BRODY HOWARD (US)
Application Number:
PCT/US2004/010126
Publication Date:
January 06, 2005
Filing Date:
March 31, 2004
Export Citation:
Assignee:
NOVOZYMES BIOTECH INC (US)
CONNELLY MARIAH (US)
BRODY HOWARD (US)
CONNELLY MARIAH (US)
BRODY HOWARD (US)
International Classes:
C07K14/37; C07K14/38; C07K14/40; C12N9/00; C12N15/52; C12P1/02; C12P21/02; (IPC1-7): C12P21/06; C12N1/14; C12N1/16; C12N1/18
Foreign References:
| US6432671B1 | 2002-08-13 | |||
| US20040142364A1 | 2004-07-22 | |||
| US20030162218A1 | 2003-08-28 |
Other References:
See also references of EP 1613761A4
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