Title:
METHYLATION-MEDIATED ADAPTER REMOVAL ON NUCLEIC ACID SEQUENCES
Document Type and Number:
WIPO Patent Application WO/2023/172520
Kind Code:
A3
Abstract:
Restriction enzymes or restriction endonucleases are enzymes that cleave DNA into fragments at recognition sites known as restriction sites. Restriction enzymes, generally found in bacteria and archaea, provide an innate immune response that cleaves foreign DNA via restriction digestion. Provided herein are methods, systems, and compositions for efficient nucleic acid assembly with improved representation and distribution. Provided herein are methods, systems, and compositions for efficient nucleic acid assembly of nucleic acids encoding genes for use in various downstream processes.
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Inventors:
CHEN SIYUAN (US)
LEE XUAN (US)
LIN DAVID (US)
RAMIREZ DANIEL (US)
GILL TAVNEET (US)
CHERRY PATRICK (US)
TORO ESTEBAN (US)
LEE XUAN (US)
LIN DAVID (US)
RAMIREZ DANIEL (US)
GILL TAVNEET (US)
CHERRY PATRICK (US)
TORO ESTEBAN (US)
Application Number:
PCT/US2023/014651
Publication Date:
November 02, 2023
Filing Date:
March 06, 2023
Export Citation:
Assignee:
TWIST BIOSCIENCE CORP (US)
International Classes:
C12Q1/683; C12N9/22; C12N15/66; C12Q1/686
Domestic Patent References:
| WO1998031838A1 | 1998-07-23 |
Foreign References:
| US20180305737A1 | 2018-10-25 | |||
| US10053688B2 | 2018-08-21 |
Other References:
CHENG ANDREW Y: "Sharpening new scissors: Designing a CRISPR-Cas9 construct for inducible and interchangeable HSATII expression in Tig-1 fibroblasts", BACHELOR'S THESIS, SWARTHMORE COLLEGE, 1 January 2021 (2021-01-01), XP093107306, Retrieved from the Internet [retrieved on 20231130]
PRYOR JOHN M., POTAPOV VLADIMIR, KUCERA REBECCA B., BILOTTI KATHARINA, CANTOR ERIC J., LOHMAN GREGORY J. S.: "Enabling one-pot Golden Gate assemblies of unprecedented complexity using data-optimized assembly design", PLOS ONE, vol. 15, no. 9, 2 September 2020 (2020-09-02), pages e0238592, XP055875130, DOI: 10.1371/journal.pone.0238592
PRYOR JOHN M., POTAPOV VLADIMIR, KUCERA REBECCA B., BILOTTI KATHARINA, CANTOR ERIC J., LOHMAN GREGORY J. S.: "Enabling one-pot Golden Gate assemblies of unprecedented complexity using data-optimized assembly design", PLOS ONE, vol. 15, no. 9, 2 September 2020 (2020-09-02), pages e0238592, XP055875130, DOI: 10.1371/journal.pone.0238592
Attorney, Agent or Firm:
REED, Sean (US)
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