Title:
MICROBIAL CULTURE METHOD AND METHOD FOR PRODUCING MICROBIAL METABOLITE
Document Type and Number:
WIPO Patent Application WO/2013/057962
Kind Code:
A1
Abstract:
Provided is a method for the efficient industrial-scale production of microorganisms and the manufacture of a microbial metabolite by microorganisms, using a carbon source having a melting point higher than the culture temperature and low solubility in water at 25°C. A microbial culture method including a step for adjusting the specific surface area of a carbon source having a melting point higher than the culture temperature and solubility of 10 g/L or lower in water at 25°C, to 20 cm2/g or higher, and a step for culturing microorganisms in the presence of this carbon source. Additionally, a method for producing a microbial metabolite, the method comprising acquiring a microbial metabolite produced by these microorganisms after culturing microorganisms by this culture method.
Inventors:
SATO SHUNSUKE
NAKAJIMA JUN
MATSUMOTO KEIJI
NAKAJIMA JUN
MATSUMOTO KEIJI
Application Number:
PCT/JP2012/006741
Publication Date:
April 25, 2013
Filing Date:
October 22, 2012
Export Citation:
Assignee:
KANEKA CORP (JP)
International Classes:
C12N1/00; C12P7/62; C12N1/16; C12N1/20
Foreign References:
| JP2003000292A | 2003-01-07 | |||
| JP2002503090A | 2002-01-29 |
Other References:
CHARLES F. BUDDE ET AL.: "Growth and polyhydroxybutyrate production by Ralstonia eutropha in emulsified plant oil medium", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 89, no. 5, March 2011 (2011-03-01), pages 1611 - 1619, XP019880871
REGINA V. ANTONIO ET AL.: "Analysis of in vivo substrate specificity of the PHA synthase from Ralstonia eutropha: formation of novel copolyesters in recombinant Escherichia coli", FEMS MICROBIOLOGY LETTERS, vol. 182, no. 1, 2000, pages 111 - 117, XP002979705
UTA BREUER ET AL.: "Yeasts as Producers of Polyhydroxyalkanoates: Genetic Engineering of Saccharomyces cerevisiae", MACROMOLECULAR BIOSCIENCE, vol. 2, no. 8, 2002, pages 380 - 386, XP002446343
REGINA V. ANTONIO ET AL.: "Analysis of in vivo substrate specificity of the PHA synthase from Ralstonia eutropha: formation of novel copolyesters in recombinant Escherichia coli", FEMS MICROBIOLOGY LETTERS, vol. 182, no. 1, 2000, pages 111 - 117, XP002979705
UTA BREUER ET AL.: "Yeasts as Producers of Polyhydroxyalkanoates: Genetic Engineering of Saccharomyces cerevisiae", MACROMOLECULAR BIOSCIENCE, vol. 2, no. 8, 2002, pages 380 - 386, XP002446343
Attorney, Agent or Firm:
PATENT CORPORATE BODY ARCO PATENT OFFICE (JP)
Patent business corporation Owner old patent firm (JP)
Patent business corporation Owner old patent firm (JP)
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