Title:
MODIFIED COLLAGEN PROTEIN AND APPLICATION OF SAME
Document Type and Number:
WIPO Patent Application WO/2019/098246
Kind Code:
A1
Abstract:
In order to develop tools and methods useful in a variety of applications, including the research and development of medical treatments which involve the modification of collagen protein and use of the same, the present invention provides a modified collagen protein expressed in a transformed cell and capable of forming collagen fibers outside of said cell, wherein the transformation is performed by introducing, into the cell, into the cell, polynucleotides coding the modified collagen protein.
Inventors:
MIWA YOSHIHIRO (JP)
KIJIMA JUNKO (JP)
KIJIMA JUNKO (JP)
Application Number:
PCT/JP2018/042181
Publication Date:
May 23, 2019
Filing Date:
November 14, 2018
Export Citation:
Assignee:
UNIV TSUKUBA (JP)
International Classes:
C12N15/62; A01K67/027; A61K38/39; A61K49/00; A61P43/00; C07K14/78; C07K19/00; C12M3/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12P21/00
Domestic Patent References:
| WO2016152882A1 | 2016-09-29 |
Foreign References:
| JP2000506362A | 2000-05-30 | |||
| JP2000125872A | 2000-05-09 | |||
| JP2008067655A | 2008-03-27 | |||
| JP2010085108A | 2010-04-15 | |||
| JP2014076026A | 2014-05-01 |
Other References:
THEIN, M. C. ET AL.: "Caenorhabditis elegans Exoskeleton Collagen COL-19: An Adult-Specific Marker for Collagen Modification and Assembly, and the analysis of Organismal Morphology", DEVELOPMENTAL DYNAMICS, vol. 226, 2003, pages 523 - 39, XP055315687, DOI: doi:10.1002/dvdy.10259
MAYE, P. ET AL.: "Generation and Characterization of Co1110al-mCherry Reporter Mice", GENESIS, vol. 49, 2011, pages 410 - 8, XP055315689, DOI: doi:10.1002/dvg.20733
OHANA, R. F. ET AL.: "HaloTag7: A genetically engineered tag that enhances bacterial expression of soluble proteins and improves protein purification", PROTEIN EXPRESSION AND PURIFICATION, vol. 68, 2009, pages 110 - 120, XP026458909, DOI: doi:10.1016/j.pep.2009.05.010
NOMURA JUN: "Cell response to mechanical stretch- Molecular cytological review", BULLETIN OF THE FACULTY OF EDUCATION, CHIBA UNIVERSITY, vol. 54, 2006, pages 271 - 274
MAYE, P. ET AL.: "Generation and Characterization of Co1110al-mCherry Reporter Mice", GENESIS, vol. 49, 2011, pages 410 - 8, XP055315689, DOI: doi:10.1002/dvg.20733
OHANA, R. F. ET AL.: "HaloTag7: A genetically engineered tag that enhances bacterial expression of soluble proteins and improves protein purification", PROTEIN EXPRESSION AND PURIFICATION, vol. 68, 2009, pages 110 - 120, XP026458909, DOI: doi:10.1016/j.pep.2009.05.010
NOMURA JUN: "Cell response to mechanical stretch- Molecular cytological review", BULLETIN OF THE FACULTY OF EDUCATION, CHIBA UNIVERSITY, vol. 54, 2006, pages 271 - 274
Attorney, Agent or Firm:
KOBAYASHI Hiroshi et al. (JP)
Download PDF: