Title:
MODIFIED CRISPR-BASED GENE EDITING SYSTEM AND METHODS OF USE
Document Type and Number:
WIPO Patent Application WO/2023/169482
Kind Code:
A1
Abstract:
Disclosed herein are systems comprising one or more modified single-guide RNAs (sgRNAs) and a donor DNA, wherein each of the modified sgRNAs comprises one or more internal anchors that are at least 5 nucleotides away from both 3' and 5' ends of each of the modified sgRNAs, wherein the donor DNA comprises one or more binding segments capable of binding to an internal anchor of the one or more internal anchors. Further disclosed herein are methods of using the systems described here.
Inventors:
ZHENG ZONGLI (CN)
WANG BANG (CN)
YU MIAO (CN)
WANG BANG (CN)
YU MIAO (CN)
Application Number:
PCT/CN2023/080356
Publication Date:
September 14, 2023
Filing Date:
March 08, 2023
Export Citation:
Assignee:
GENEDITBIO LTD (CN)
International Classes:
C12N15/113; C12N9/22; C12N15/10; C12N15/63
Foreign References:
| CN114072498A | 2022-02-18 | |||
| CN112088018A | 2020-12-15 | |||
| US20190161742A1 | 2019-05-30 | |||
| US20180127785A1 | 2018-05-10 |
Other References:
MALI, P ET AL.: "Cas9 as a versatile tool for engineering biology", NATURE METHODS, vol. 10, no. 10, 31 October 2013 (2013-10-31), XP055553170, DOI: 10.1038/nmeth.2649
GUOLING LI ET AL.: "Recent developments in enhancing the efficiency of CRISPR/Cas9- mediated knock-in in animals", HEREDITAS, vol. 42, no. 7, 2 June 2020 (2020-06-02), pages 641 - 656, XP009548697
GUOLING LI ET AL.: "Recent developments in enhancing the efficiency of CRISPR/Cas9- mediated knock-in in animals", HEREDITAS, vol. 42, no. 7, 2 June 2020 (2020-06-02), pages 641 - 656, XP009548697
Attorney, Agent or Firm:
AFD CHINA INTELLECTUAL PROPERTY LAW OFFICE (CN)
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