Title:
MODIFIED GRANULOCYTE COLONY STIMULATING FACTOR (G-CSF) WITH REDUCED IMMUNOGENICITY
Document Type and Number:
WIPO Patent Application WO2002077034
Kind Code:
A3
Abstract:
The present invention relates to polypeptides to be administered especially to humans and in particular for therapeutic use. The polypeptides are modified polypeptides whereby the modification results in a reduced propensity for the polypeptide to elicit an immune response upon administration to the human subject. The invention in particular relates to the modification of granulocyte colony stimulating factor (G-CSF) to result in granulocyte colony stimulating factor (G-CSF) proteins that are substantially non-immunogenic or less immunogenic than any non-modified counterpart when used in vivo.
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Inventors:
CARR FRANCIS J (GB)
CARTER GRAHAM (GB)
JONES TIM (GB)
WILLIAMS STEPHEN (GB)
CARTER GRAHAM (GB)
JONES TIM (GB)
WILLIAMS STEPHEN (GB)
Application Number:
PCT/EP2002/001171
Publication Date:
December 11, 2003
Filing Date:
February 05, 2002
Export Citation:
Assignee:
MERCK PATENT GMBH (DE)
CARR FRANCIS J (GB)
CARTER GRAHAM (GB)
JONES TIM (GB)
WILLIAMS STEPHEN (GB)
CARR FRANCIS J (GB)
CARTER GRAHAM (GB)
JONES TIM (GB)
WILLIAMS STEPHEN (GB)
International Classes:
A61K9/70; A61K38/00; A61K38/18; A61K47/02; A61K47/12; C12N15/09; A61K48/00; A61P37/02; C07K7/06; C07K7/08; C07K14/535; C07K14/54; C07K16/18; C07K16/28; C07K16/30; C07K16/46; C12N15/27; C12P21/02; G16B15/20; G16B20/00; (IPC1-7): C07K14/535; A61K38/18; C12N15/27; C07K7/06; C07K7/08
Domestic Patent References:
| WO2000034317A2 | 2000-06-15 | |||
| WO1998052976A1 | 1998-11-26 | |||
| WO1998059244A1 | 1998-12-30 |
Foreign References:
| US5824784A | 1998-10-20 | |||
| EP0272703A1 | 1988-06-29 |
Other References:
LARICCHIA-ROBBIO LEOPOLDO ET AL: "Naturally occurring and therapy-induced antibodies to human granulocyte colony-stimulating factor (G-CSF) in human serum.", JOURNAL OF CELLULAR PHYSIOLOGY, vol. 173, no. 2, November 1997 (1997-11-01), pages 219 - 226, XP002206160, ISSN: 0021-9541
SALI A ET AL: "COMPARATIVE PROTEIN MODELLING BY SATISFACTION OF SPATIAL RESTRAINTS", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 234, 1993, pages 779 - 815, XP002948615, ISSN: 0022-2836
ALTUVIA Y ET AL: "RANKING POTENTIAL BINDING PEPTIDES TO MHC MOLECULES BY A COMPUTATIONAL THREADING APPROACH", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 249, no. 1, 1995, pages 244 - 250, XP000925520, ISSN: 0022-2836
BRUSIC VLADIMIR ET AL: "MHCPEP, a database of MHC-binding peptides: Update 1997.", NUCLEIC ACIDS RESEARCH, vol. 26, no. 1, 1 January 1998 (1998-01-01), pages 368 - 371, XP002204283, ISSN: 0305-1048
BOEHM H-J: "PREDICTION OF BINDING CONSTANTS OF PROTEIN LIGANDS: A FAST METHOD FOR THE PRIORITIZATION OF HITS OBTAINED FROM DE NOVO DESIGN OR 3D DATABASE SEARCH PROGRAMS", JOURNAL OF COMPUTER-AIDED MOLECULAR DESIGN, ESCOM SCIENCE PUBLISHERS BV, XX, vol. 4, no. 12, July 1998 (1998-07-01), pages 309 - 323, XP008004781, ISSN: 0920-654X
SALI A ET AL: "COMPARATIVE PROTEIN MODELLING BY SATISFACTION OF SPATIAL RESTRAINTS", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 234, 1993, pages 779 - 815, XP002948615, ISSN: 0022-2836
ALTUVIA Y ET AL: "RANKING POTENTIAL BINDING PEPTIDES TO MHC MOLECULES BY A COMPUTATIONAL THREADING APPROACH", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 249, no. 1, 1995, pages 244 - 250, XP000925520, ISSN: 0022-2836
BRUSIC VLADIMIR ET AL: "MHCPEP, a database of MHC-binding peptides: Update 1997.", NUCLEIC ACIDS RESEARCH, vol. 26, no. 1, 1 January 1998 (1998-01-01), pages 368 - 371, XP002204283, ISSN: 0305-1048
BOEHM H-J: "PREDICTION OF BINDING CONSTANTS OF PROTEIN LIGANDS: A FAST METHOD FOR THE PRIORITIZATION OF HITS OBTAINED FROM DE NOVO DESIGN OR 3D DATABASE SEARCH PROGRAMS", JOURNAL OF COMPUTER-AIDED MOLECULAR DESIGN, ESCOM SCIENCE PUBLISHERS BV, XX, vol. 4, no. 12, July 1998 (1998-07-01), pages 309 - 323, XP008004781, ISSN: 0920-654X
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