Title:
MONOCLONAL ANTIBODY PRODUCTION BY EBV TRANSFORMATION OF B CELLS
Document Type and Number:
WIPO Patent Application WO2004076677
Kind Code:
A3
Abstract:
A method for producing a clone of an immortalised human B memory lymphocyte, comprising the step of transforming human B memory lymphocytes using Epstein Barr Virus (EBV) in the presence of a polyclonal B cell activator. The method is particularly useful in a method for producing a clone of an immortalised human B memory lymphocyte capable of producing a human monoclonal antibody with a desired antigen specificity, comprising the steps of: (i) selecting and isolating a human memory B lymphocyte subpopulation; (ii) transforming the subpopulation with Epstein Barr Virus (EBV) in the presence of a polyclonal B cell activator; (iii) screening the culture supernatant for antigen specificity; and (iv) isolating an immortalised human B memory lymphocyte clone capable of producing a human monoclonal antibody having the desired antigen specificity.
Inventors:
LANZAVECCHIA ANTONIO (CH)
Application Number:
PCT/IB2004/001071
Publication Date:
March 31, 2005
Filing Date:
February 26, 2004
Export Citation:
Assignee:
INST RESEARCH IN BIOMEDICINE (CH)
LANZAVECCHIA ANTONIO (CH)
LANZAVECCHIA ANTONIO (CH)
International Classes:
A61K39/395; A61P31/14; C07K16/00; C07K16/10; C07K16/12; C07K16/20; G01N33/49; C12Q; (IPC1-7): C07K16/00; C07K16/10; C07K16/20; C07K16/12; A61K39/395; G01N33/49; A61P31/14
Foreign References:
| US4997764A | 1991-03-05 |
Other References:
BABA T W ET AL: "Human neutralizing monoclonal antibodies of the IgG1 subtype protect against mucosal simian-human immunodeficiency virus infection.", NATURE MEDICINE. FEB 2000, vol. 6, no. 2, February 2000 (2000-02-01), pages 200 - 206, XP002296336, ISSN: 1078-8956
NIEDBALA W G ET AL: "A COMPARISON OF THREE METHODS FOR PRODUCTION OF HUMAN HYBRIDOMAS SECRETING AUTOANTIBODIES", HYBRIDOMA, LIEBERT, NEW YORK, NY, US, vol. 17, no. 3, June 1998 (1998-06-01), pages 299 - 304, XP001015996, ISSN: 0272-457X
MULDER A ET AL: "A HUMAN MONOCLONAL ANTIBODY, PRODUCED FOLLOWING IN VITRO IMMUNIZATION, RECOGNIZING AN EPITOPE SHARED BY HLA-A2 SUBTYPES AND HLA-A28", TISSUE ANTIGENS, MUNKSGAARD, COPENHAGEN, DK, vol. 42, no. 1, July 1993 (1993-07-01), pages 27 - 34, XP001015992, ISSN: 0001-2815
DROSTEN CHRISTIAN ET AL: "Identification of a novel coronavirus in patients with severe acute respiratory syndrome", NEW ENGLAND JOURNAL OF MEDICINE, MASSACHUSETTS MEDICAL SOCIETY, BOSTON, MA, US, vol. 348, no. 20, 10 April 2003 (2003-04-10), pages 1967 - 1976, XP002288120, ISSN: 1533-4406
NIEDBALA W G ET AL: "A COMPARISON OF THREE METHODS FOR PRODUCTION OF HUMAN HYBRIDOMAS SECRETING AUTOANTIBODIES", HYBRIDOMA, LIEBERT, NEW YORK, NY, US, vol. 17, no. 3, June 1998 (1998-06-01), pages 299 - 304, XP001015996, ISSN: 0272-457X
MULDER A ET AL: "A HUMAN MONOCLONAL ANTIBODY, PRODUCED FOLLOWING IN VITRO IMMUNIZATION, RECOGNIZING AN EPITOPE SHARED BY HLA-A2 SUBTYPES AND HLA-A28", TISSUE ANTIGENS, MUNKSGAARD, COPENHAGEN, DK, vol. 42, no. 1, July 1993 (1993-07-01), pages 27 - 34, XP001015992, ISSN: 0001-2815
DROSTEN CHRISTIAN ET AL: "Identification of a novel coronavirus in patients with severe acute respiratory syndrome", NEW ENGLAND JOURNAL OF MEDICINE, MASSACHUSETTS MEDICAL SOCIETY, BOSTON, MA, US, vol. 348, no. 20, 10 April 2003 (2003-04-10), pages 1967 - 1976, XP002288120, ISSN: 1533-4406
Download PDF:
Previous Patent: GLUTAMATE TRANSPORT MODULATORY COMPOUNDS AND METHODS
Next Patent: TRANSLUCENT SOLID MATRIX ASSAY DEVICE FOR MICROARRAY ANALYSIS
Next Patent: TRANSLUCENT SOLID MATRIX ASSAY DEVICE FOR MICROARRAY ANALYSIS