MUTATED DNA POLYMERASES WITH INCREASED MISPAIRING DISCRIMINATION

WIPO Patent Application WO2005074350

A3

The invention relates to DNA polymerases with a special mutation, exhibiting increased mispairing discrimination. The invention also relates to the production and use thereof. The thermostable DNA polymerases with said mutation are particularly suitable for diagnostic and molecular biological methods, e.g. allele-specific PCR.

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MARX ANDREAS (DE)
SUMMERER DANIEL (DE)
RUDINGER NICOLAUS ZACKES (DE)

PCT/EP2005/050479

November 24, 2005

February 04, 2005

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UNIV BONN (DE)
MARX ANDREAS (DE)
SUMMERER DANIEL (DE)
RUDINGER NICOLAUS ZACKES (DE)

C12N9/12; C12Q1/68; (IPC1-7): C12N9/12

MINNICK T ET AL.: "side chains that influence fidelity at the polymerase active site of Escherichia coli DNA polymerase I (klenow fragment)", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 275, no. 5, 29 January 1999 (1999-01-29), pages 3067 - 3075, XP002341285
PAVLOV A. ET AL: "Recent developments in the optimization of thermostable DNA polymerases for efficient applications", TRENDS IN BIOTECHNOLOGY, vol. 22, no. 5, May 2004 (2004-05-01), pages 253 - 259, XP004504365
PATEL P. ET AL.: "Prokaryotic DNA polymerase I: evolution, structure, and "base flipping" mechanism for nucleotide selection", J. MOL. BIOL., vol. 308, 2001, pages 823 - 837, XP004466165

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