Title:
NEAR-INFRARED GASTRIC CANCER TEST KIT AND PREPARATION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2024/060432
Kind Code:
A1
Abstract:
A near-infrared gastric cancer test kit and a preparation method therefor. Under the action of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS), a near-infrared fluorescence reagent aminated indocyanine green (ICG-NH2) is covalently coupled to a gastric cancer cell Her-2 monoclonal antibody (Her-2 mAb) by means of an amidation reaction, to form a novel gastric cancer cell test probe having near-infrared fluorescence characteristics. By means of the specific binding of Her-2 mAb to gastric cancer cells, ICG binds to the surface of gastric cancer cells or tumor tissue cells. By means of a fluorescence microscope or a laser confocal microscope and under excitation of a specific wavelength, rapid imaging test of gastric cancer cells and tumor tissues can be implemented. The kit has the characteristics of strong specificity, high sensitivity, and high resolution.
Inventors:
CUI DAXIANG (CN)
PENG JIAWEI (CN)
WANG QING (CN)
CUI MINGQING (CN)
ZHOU CHENG (CN)
LI XUELING (CN)
WANG DAN (CN)
CHEN XIAOMIN (CN)
LIU ZEXI (CN)
PENG JIAWEI (CN)
WANG QING (CN)
CUI MINGQING (CN)
ZHOU CHENG (CN)
LI XUELING (CN)
WANG DAN (CN)
CHEN XIAOMIN (CN)
LIU ZEXI (CN)
Application Number:
PCT/CN2022/139575
Publication Date:
March 28, 2024
Filing Date:
December 16, 2022
Export Citation:
Assignee:
SHANGHAI NATIONAL ENGINEERING RES CENTER FOR NANOTECHNOLOGY LTD (CN)
International Classes:
G01N21/359; G01N21/3577
Domestic Patent References:
| WO2020160006A1 | 2020-08-06 | |||
| WO2010098435A1 | 2010-09-02 |
Foreign References:
| CN114199655A | 2022-03-18 | |||
| CN109121429A | 2019-01-01 | |||
| CN110791281A | 2020-02-14 |
Other References:
JEONG KYOUNGYUN, KONG SEONG-HO, BAE SEONG-WOO, PARK CHO RONG, BERLTH FELIX, SHIN JAE HWAN, LEE YUN-SANG, YOUN HYEWON, KOO EUNHEE, : "Evaluation of Near-infrared Fluorescence-conjugated Peptides for Visualization of Human Epidermal Receptor 2-overexpressed Gastric Cancer", JOURNAL OF GASTRIC CANCER, vol. 21, no. 2, 1 January 2021 (2021-01-01), pages 191, XP093150630, ISSN: 2093-582X, DOI: 10.5230/jgc.2021.21.e18
Attorney, Agent or Firm:
SHANGHAI EAST ASIA PATENT & TRADEMARK AGENCY CO., LTD. (CN)
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