Title:
NEURITE OUTGROWTH PROMOTION KIT AND USE THEREFOR
Document Type and Number:
WIPO Patent Application WO/2022/250127
Kind Code:
A1
Abstract:
The present invention pertains to: a neurite outgrowth promotion kit which contains a γ-secretase inhibitor or a GADD45G expression vector, and significantly promotes, in comparison to a control, neurite outgrowth in nerve cells by acting on a neurosphere so as to cause induced differentiation into nerve cells; a method for producing a neurosphere for treatment of spinal cord injuries which involves a step for applying the neurite outgrowth promotion kit to a neurosphere derived from pluripotent stem cells; a neurosphere for treatment of spinal cord injuries; and a method for selecting a neurosphere for treatment of spinal cord injuries and a method for screening neurite outgrowth promoters.
Inventors:
OKANO HIDEYUKI (JP)
KASE YOSHITAKA (JP)
KASE YOSHITAKA (JP)
Application Number:
PCT/JP2022/021676
Publication Date:
December 01, 2022
Filing Date:
May 27, 2022
Export Citation:
Assignee:
UNIV KEIO (JP)
International Classes:
C12N5/079; A61K35/30; A61L27/38; C12N15/63; C12Q1/02
Foreign References:
| US20110129450A1 | 2011-06-02 | |||
| JP2021084882A | 2021-06-03 | |||
| US20190241869A1 | 2019-08-08 | |||
| JP2021089367A | 2021-06-10 |
Other References:
OKUBO TOSHIKI, NAGOSHI NARIHITO, KOHYAMA JUN, TSUJI OSAHIKO, SHINOZAKI MUNEHISA, SHIBATA SHINSUKE, KASE YOSHITAKA, MATSUMOTO MORIO: "Treatment with a Gamma-Secretase Inhibitor Promotes Functional Recovery in Human iPSC- Derived Transplants for Chronic Spinal Cord Injury", STEM CELL REPORTS, CELL PRESS, UNITED STATES, vol. 11, no. 6, 1 December 2018 (2018-12-01), United States , pages 1416 - 1432, XP093007589, ISSN: 2213-6711, DOI: 10.1016/j.stemcr.2018.10.022
KASE YOSHITAKA, SATO TSUKIKA, OKANO YUJI, OKANO HIDEYUKI: "The GADD45G/p38 MAPK/CDC25B signaling pathway enhances neurite outgrowth by promot- ing microtubule polymerization", ISCIENCE, vol. 25, no. 4, 15 April 2022 (2022-04-15), pages e104089, XP093007587, DOI: 10.1016/j.isci.2022.104089
KEIRSTEAD H. S. ET AL.: "Human Embryonic Stem Cell-Derived Oligodendrocyte Progenitor Cell Transplants Remyelinate and Restore Locomotion after Spinal Cord Injury", J. NEUROSCI., vol. 25, no. 19, 2005, pages 4694 - 4705, XP055093017, DOI: 10.1523/JNEUROSCI.0311-05.2005
OKUBO T. ET AL.: "Treatment with a Gamma-Secretase Inhibitor Promotes Functional Recovery in Human iPSC- Derived Transplants for Chronic Spinal Cord Injury", STEM CELL REPORTS, vol. 11, no. 6, 2018, pages 1416 - 1432, XP093007589, DOI: 10.1016/j.stemcr.2018.10.022
KASE YOSHITAKA, SATO TSUKIKA, OKANO YUJI, OKANO HIDEYUKI: "The GADD45G/p38 MAPK/CDC25B signaling pathway enhances neurite outgrowth by promot- ing microtubule polymerization", ISCIENCE, vol. 25, no. 4, 15 April 2022 (2022-04-15), pages e104089, XP093007587, DOI: 10.1016/j.isci.2022.104089
KEIRSTEAD H. S. ET AL.: "Human Embryonic Stem Cell-Derived Oligodendrocyte Progenitor Cell Transplants Remyelinate and Restore Locomotion after Spinal Cord Injury", J. NEUROSCI., vol. 25, no. 19, 2005, pages 4694 - 4705, XP055093017, DOI: 10.1523/JNEUROSCI.0311-05.2005
OKUBO T. ET AL.: "Treatment with a Gamma-Secretase Inhibitor Promotes Functional Recovery in Human iPSC- Derived Transplants for Chronic Spinal Cord Injury", STEM CELL REPORTS, vol. 11, no. 6, 2018, pages 1416 - 1432, XP093007589, DOI: 10.1016/j.stemcr.2018.10.022
Attorney, Agent or Firm:
TAZAKI Akira et al. (JP)
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