Title:
NUCLEIC ACID DELIVERY VEHICLE
Document Type and Number:
WIPO Patent Application WO1997010851
Kind Code:
A3
Abstract:
A composition includes a liposome which has a polynucleic acid and a peptide capable of disrupting membranes under acidic conditions encapsulated within it. The composition is used for efficient transfer of nucleic acids into cells both in vitro as well as in vivo.
Inventors:
BARU MOSHE (IL)
NUR ISRAEL (IL)
NUR ISRAEL (IL)
Application Number:
PCT/IL1996/000101
Publication Date:
June 05, 1997
Filing Date:
September 05, 1996
Export Citation:
Assignee:
OPPERBAS HOLDING BV (NL)
BARU MOSHE (NL)
NUR ISRAEL (NL)
BARU MOSHE (NL)
NUR ISRAEL (NL)
International Classes:
A61K9/127; A61K38/37; C12N15/09; A61K38/48; A61K47/14; A61K47/42; A61K48/00; C07K7/04; C12N5/10; (IPC1-7): A61K48/00; A61K9/127
Domestic Patent References:
| WO1985004880A1 | 1985-11-07 | |||
| WO1993007283A1 | 1993-04-15 | |||
| WO1995021259A1 | 1995-08-10 | |||
| WO1991006309A1 | 1991-05-16 |
Other References:
C. PLANK ET AL: "The Influence of Endosome-disruptive Peptides on Gene Transfer Using Synthetic Virus-like Gene Transfer Systems", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 269, no. 17, 29 April 1994 (1994-04-29), USA, pages 12918 - 12924, XP000615488
R. J. MANNINO ET AL: "Liposome mediated gene transfer", BIOTECHNIQUES, vol. 6, no. 7, 1988, pages 682 - 690, XP000602881
E. WAGNER ET AL: "Influenza virus hemagglutinin HA-2 N-terminal fusogenic peptides augment gene transfer by transferrin-polylysine-DNA complexes: Toward a synthetic virus-like gene-transfer vehicle", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 89, September 1992 (1992-09-01), USA, pages 7934 - 7938, XP000371760
D. T. CURIEL ET AL: "Adenovirus enhancement of transferrin-polylysine-mediated gene delivery", PROCCEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 88, October 1991 (1991-10-01), USA, pages 8850 - 8854, XP000569906
R. J. MANNINO ET AL: "Liposome mediated gene transfer", BIOTECHNIQUES, vol. 6, no. 7, 1988, pages 682 - 690, XP000602881
E. WAGNER ET AL: "Influenza virus hemagglutinin HA-2 N-terminal fusogenic peptides augment gene transfer by transferrin-polylysine-DNA complexes: Toward a synthetic virus-like gene-transfer vehicle", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 89, September 1992 (1992-09-01), USA, pages 7934 - 7938, XP000371760
D. T. CURIEL ET AL: "Adenovirus enhancement of transferrin-polylysine-mediated gene delivery", PROCCEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 88, October 1991 (1991-10-01), USA, pages 8850 - 8854, XP000569906
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