Title:
NUCLEIC ACID FRAGMENT AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/176910
Kind Code:
A1
Abstract:
A nucleic acid fragment including a polymerase binding sequence and a transposase binding sequence, the polymerase binding sequence or the transposase binding sequence including a caged nucleotide residue; a specific binding substance; a method for binding the polymerase or the transposase to a nucleic acid fragment; a method for inserting a nucleic acid fragment in the vicinity of a binding region of a DNA-binding protein bound to a DNA molecule; a method for gene amplification of a DNA molecule into which a nucleic acid fragment has been inserted.
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Inventors:
OHKAWA YASUYUKI (JP)
HARADA AKIHITO (JP)
OKI SHINYA (JP)
HARADA AKIHITO (JP)
OKI SHINYA (JP)
Application Number:
PCT/JP2022/006187
Publication Date:
August 25, 2022
Filing Date:
February 16, 2022
Export Citation:
Assignee:
UNIV KYUSHU NAT UNIV CORP (JP)
International Classes:
C12N15/11; C07K16/00; C12Q1/686; C12Q1/6865; C12Q1/6869
Domestic Patent References:
WO2020235563A1 | 2020-11-26 | |||
WO2018042776A1 | 2018-03-08 | |||
WO2018042776A1 | 2018-03-08 |
Foreign References:
US20170233722A1 | 2017-08-17 | |||
JP2021025468A | 2021-02-22 |
Attorney, Agent or Firm:
TAZAKI Akira et al. (JP)
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